PXD015751 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cellular interactome dynamics during paclitaxel treatment |
Description | Cell cycle inhibitors, including paclitaxel, are among the most widely used and effective cancer therapies. However, several challenges limit the success of paclitaxel including drug resistance and toxic side effects. Paclitaxel is thought to act primarily by stabilizing microtubules and locking cells in a mitotic state. However, the resulting cytotoxicity and tumor shrinkage rates observed cannot be fully explained by this mechanism alone. Here we apply quantitative chemical cross-linking with mass spectrometry analysis to paclitaxel treated cells. Our results provide large scale measurements of relative protein levels and, perhaps more importantly, changes to protein conformations and interactions that occur upon paclitaxel treatment. Drug concentration dependent changes are revealed in known drug targets including tubulins, as well as many other proteins and protein complexes involved in apoptotic signaling and cellular homeostasis. As such this study provides insight into systems-level changes to protein structures and interactions which occur with paclitaxel treatment. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-21 |
AnnouncementXML | Submission_2019-11-21_03:40:28.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Juan Chavez |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ FT; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-09 01:23:37 | ID requested | |
⏵ 1 | 2019-11-21 03:40:29 | announced | |
2 | 2020-01-14 07:08:10 | announced | 2020-01-14: Updated publication reference for PubMed record(s): 31747606. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: chemical cross-linking, mitotic inhibitor |
Contact List
James E. Bruce |
contact affiliation | Genome Sciences, University of Washington, Seattle WA, USA |
contact email | jimbruce@uw.edu |
lab head | |
Juan Chavez |
contact affiliation | University of Washington |
contact email | jdchavez@uw.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015751
- Label: PRIDE project
- Name: Cellular interactome dynamics during paclitaxel treatment