PXD015751

PXD015751 is an original dataset announced via ProteomeXchange.

Title	Cellular interactome dynamics during paclitaxel treatment
Description	Cell cycle inhibitors, including paclitaxel, are among the most widely used and effective cancer therapies. However, several challenges limit the success of paclitaxel including drug resistance and toxic side effects. Paclitaxel is thought to act primarily by stabilizing microtubules and locking cells in a mitotic state. However, the resulting cytotoxicity and tumor shrinkage rates observed cannot be fully explained by this mechanism alone. Here we apply quantitative chemical cross-linking with mass spectrometry analysis to paclitaxel treated cells. Our results provide large scale measurements of relative protein levels and, perhaps more importantly, changes to protein conformations and interactions that occur upon paclitaxel treatment. Drug concentration dependent changes are revealed in known drug targets including tubulins, as well as many other proteins and protein complexes involved in apoptotic signaling and cellular homeostasis. As such this study provides insight into systems-level changes to protein structures and interactions which occur with paclitaxel treatment.
HostingRepository	PRIDE
AnnounceDate	2019-11-21
AnnouncementXML	Submission_2020-01-14_07:07:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Chavez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-09 01:23:37	ID requested
1	2019-11-21 03:40:29	announced
⏵ 2	2020-01-14 07:08:10	announced	2020-01-14: Updated publication reference for PubMed record(s): 31747606.

Chavez JD, Keller A, Zhou B, Tian R, Bruce JE, Cellular Interactome Dynamics during Paclitaxel Treatment. Cell Rep, 29(8):2371-2383.e5(2019) [pubmed]

submitter keyword: chemical cross-linking, mitotic inhibitor

James E. Bruce
contact affiliation	Genome Sciences, University of Washington, Seattle WA, USA
contact email	jimbruce@uw.edu
lab head
Juan Chavez
contact affiliation	University of Washington
contact email	jdchavez@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015751
     1. Label: PRIDE project
     2. Name: Cellular interactome dynamics during paclitaxel treatment