PXD015681-1

PXD015681 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analysis reveals mechanisms of complete resistance to extreme illumination levels in a green alga isolated from desert soil crusts
Description	The unparalleled performance of Chlorella ohadii under irradiances of twice-full sun light underlines that we lack essential information on how the photosynthetic machinery operates and what sets its upper functional limit. Rather than succumbing to photodamage, C. ohadii undergoes major structural and compositional changes pointing to unique features of PSII function, and exhibiting highly efficient utilization of reductants downstream of the photosynthetic reaction centers. This remarkable resistance allowed us to investigate, for the first time, the systems response of photosynthesis and growth to extreme illumination in a metabolically active cell. Using redox proteomics, transcriptomics, metabolomics and lipidomics, we explored the cellular mechanisms underlying inorganic carbon concentration, excess redox dissipation, protein S-glutathionylation, lipid and starch accumulation and increased thylakoid stacking. Frequently, the response in C. ohadii deviated from those reported in model species, reflecting its life history in desert sand crusts. It exhibited a readily available metabolic capacity to utilize a sudden excess of reducing power to support growth and reserve formation. Redox regulation played a pivotal role in this very rapid acclimation process. Comparative global and case-specific analyses, provided insights into the potential evolutionary role of effective reductant utilization in the extreme resistance of C. ohadii to photoinhibition.
HostingRepository	PRIDE
AnnounceDate	2020-06-03
AnnouncementXML	Submission_2020-06-03_00:51:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Beata Siemiatkowska
SpeciesList	scientific name: Chlorella vulgaris (Green alga); NCBI TaxID: 3077;
ModificationList	sulfated residue; disulfide conjugated residue; N-ethylmaleimide derivatized cysteine; reduction of disulfide crosslink in cystine to two cysteines
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-02 05:28:51	ID requested
⏵ 1	2020-06-03 00:51:43	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Chlorella ohadii, redoxome, redox, proteome. light

Mark Stitt1
contact affiliation	Max-Planck Institute for Molecular Plant Physiology, Potsdam, 14476, Germany
contact email	MStitt@mpimp-golm.mpg.de
lab head
Beata Siemiatkowska
contact affiliation	Max Planck Institute of Molecular Plant Physiology
contact email	Siemiatkowska@mpimp-golm.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD015681

PRIDE project URI

• PRIDE
  1. PXD015681
     1. Label: PRIDE project
     2. Name: Multi-omics analysis reveals mechanisms of complete resistance to extreme illumination levels in a green alga isolated from desert soil crusts