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PXD015681

PXD015681 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMulti-omics analysis reveals mechanisms of complete resistance to extreme illumination levels in a green alga isolated from desert soil crusts
DescriptionThe unparalleled performance of Chlorella ohadii under irradiances of twice-full sun light underlines that we lack essential information on how the photosynthetic machinery operates and what sets its upper functional limit. Rather than succumbing to photodamage, C. ohadii undergoes major structural and compositional changes pointing to unique features of PSII function, and exhibiting highly efficient utilization of reductants downstream of the photosynthetic reaction centers. This remarkable resistance allowed us to investigate, for the first time, the systems response of photosynthesis and growth to extreme illumination in a metabolically active cell. Using redox proteomics, transcriptomics, metabolomics and lipidomics, we explored the cellular mechanisms underlying inorganic carbon concentration, excess redox dissipation, protein S-glutathionylation, lipid and starch accumulation and increased thylakoid stacking. Frequently, the response in C. ohadii deviated from those reported in model species, reflecting its life history in desert sand crusts. It exhibited a readily available metabolic capacity to utilize a sudden excess of reducing power to support growth and reserve formation. Redox regulation played a pivotal role in this very rapid acclimation process. Comparative global and case-specific analyses, provided insights into the potential evolutionary role of effective reductant utilization in the extreme resistance of C. ohadii to photoinhibition.
HostingRepositoryPRIDE
AnnounceDate2020-06-03
AnnouncementXMLSubmission_2020-06-03_00:51:42.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBeata Siemiatkowska
SpeciesList scientific name: Chlorella vulgaris (Green alga); NCBI TaxID: 3077;
ModificationListsulfated residue; disulfide conjugated residue; N-ethylmaleimide derivatized cysteine; reduction of disulfide crosslink in cystine to two cysteines
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-02 05:28:51ID requested
12020-06-03 00:51:43announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Chlorella ohadii, redoxome, redox, proteome. light
Contact List
Mark Stitt1
contact affiliationMax-Planck Institute for Molecular Plant Physiology, Potsdam, 14476, Germany
contact emailMStitt@mpimp-golm.mpg.de
lab head
Beata Siemiatkowska
contact affiliationMax Planck Institute of Molecular Plant Physiology
contact emailSiemiatkowska@mpimp-golm.mpg.de
dataset submitter
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