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PXD015650 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MSMS of DCC and Neuropilin-1 immunoprecipitated samples from Xenopus Laevis brains
DescriptionDuring neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. Here, we analyzed the interactome of endogenous DCC and Neuropilin-1 by analysing immunoprecipitated samples obtained from Xenopus Laevis brains with LC-MSMS. We find that DCC and Neuropilin-1 bind to ribosomal proteins and specific RNA-binding proteins confirming that multiple guidance cue receptors can bind to ribosomes and may use receptor-ribosome coupling to regulate cue-induced local translation in axons.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterToshiaki Shigeoka
SpeciesList scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationListmonohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-01 02:01:36ID requested
12019-12-02 02:28:54announced
Publication List
Koppers M, Cagnetta R, Shigeoka T, Wunderlich LC, Vallejo-Ramirez P, Qiaojin Lin J, Zhao S, Jakobs MA, Dwivedy A, Minett MS, Bellon A, Kaminski CF, Harris WA, Flanagan JG, Holt CE, Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. Elife, 8():(2019) [pubmed]
Keyword List
submitter keyword: DCC, Neuropilin-1, immunoprecipitation, Xenopus Laevis brains, LC-MSMS, LFQ
Contact List
Christine E. Holt
contact affiliationDepartment of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK.
contact emailceh33@cam.ac.uk
lab head
Toshiaki Shigeoka
contact affiliationUniversity of Cambridge
contact emailts561@cam.ac.uk
dataset submitter
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