PXD015650-1

PXD015650 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS of DCC and Neuropilin-1 immunoprecipitated samples from Xenopus Laevis brains
Description	During neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. Here, we analyzed the interactome of endogenous DCC and Neuropilin-1 by analysing immunoprecipitated samples obtained from Xenopus Laevis brains with LC-MSMS. We find that DCC and Neuropilin-1 bind to ribosomal proteins and specific RNA-binding proteins confirming that multiple guidance cue receptors can bind to ribosomes and may use receptor-ribosome coupling to regulate cue-induced local translation in axons.
HostingRepository	PRIDE
AnnounceDate	2019-12-02
AnnouncementXML	Submission_2019-12-02_02:28:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Toshiaki Shigeoka
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-01 02:01:36	ID requested
⏵ 1	2019-12-02 02:28:54	announced

Koppers M, Cagnetta R, Shigeoka T, Wunderlich LC, Vallejo-Ramirez P, Qiaojin Lin J, Zhao S, Jakobs MA, Dwivedy A, Minett MS, Bellon A, Kaminski CF, Harris WA, Flanagan JG, Holt CE, Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. Elife, 8():(2019) [pubmed]

submitter keyword: DCC, Neuropilin-1, immunoprecipitation, Xenopus Laevis brains, LC-MSMS, LFQ

Christine E. Holt
contact affiliation	Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK.
contact email	ceh33@cam.ac.uk
lab head
Toshiaki Shigeoka
contact affiliation	University of Cambridge
contact email	ts561@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015650

PRIDE project URI

• PRIDE
  1. PXD015650
     1. Label: PRIDE project
     2. Name: LC-MSMS of DCC and Neuropilin-1 immunoprecipitated samples from Xenopus Laevis brains