PXD015609-1

PXD015609 is an original dataset announced via ProteomeXchange.

Title	hnRNP 1 H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma
Description	RNA G-quadruplexes (RG4s) are four-stranded structures known to control mRNA translation of cancer relevant genes. RG4 formation is pervasive in vitro but not in cellulo, indicating the existence of a poorly characterized molecular machinery that remodels RG4s and maintains them unfolded. To help fill this gap in knowledge, we performed a quantitative proteomic screen to identify cytosolic proteins that interact with a canonical RG4 in its folded and unfolded conformation. Our results identified hnRNP H/F as important components of the cytoplasmic machinery modulating RG4s structural integrity, revealed their function in RG4-mediated translation and uncovered the underlying molecular mechanism impacting cellular stress response linked to the outcome of glioblastoma, one of the deadliest types of solid cancer overall.
HostingRepository	PRIDE
AnnounceDate	2020-11-11
AnnouncementXML	Submission_2020-11-11_09:03:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	François GUILLONNEAU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-26 05:34:33	ID requested
⏵ 1	2020-11-11 09:03:31	announced

Herviou P, Le Bras M, Dumas L, Hieblot C, Gilhodes J, Cioci G, Hugnot JP, Ameadan A, Guillonneau F, Dassi E, Cammas A, Millevoi S, hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma. Nat Commun, 11(1):2661(2020) [pubmed]

submitter keyword: human glioblastoma, G-quadruplex RNA-protein interactions, AP-MS, Orbitrap Fusion

Stefania Millevoi
contact affiliation	Cancer Research Center of Toulouse (CRCT) TEAM "RNA-binding proteins and post-transcriptional regulation in cancer" UMR1037
contact email	stefania.millevoi@inserm.fr
lab head
François GUILLONNEAU
contact affiliation	3P5 Université Paris Descartes
contact email	francois.guillonneau@parisdescartes.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD015609

PRIDE project URI

• PRIDE
  1. PXD015609
     1. Label: PRIDE project
     2. Name: hnRNP 1 H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma