PXD015502-1

PXD015502 is an original dataset announced via ProteomeXchange.

Title	Signaling proteins of a chemosensory pathway from sperm of Arbacia punctulata
Description	Cilia and flagella serve as cellular antennae that translate sensory information into cellular responses. In sperm, a single chemoattractant molecule triggers signaling events that evoke a Ca2+ response and chemotactic steering. Key components of the chemotactic signaling pathway from sperm of the sea urchin Arbacia punctulata were investigated. Targeted proteomics employed a spiked-in heavy isotope-labelled standard protein (QConCat) and in-gel protein digestion with trypsin. The standard protein together with the previously determined copy number of the receptor guanylate cyclase allowed determining the copy numbers for 19 polypeptides of this signaling pathway.
HostingRepository	PanoramaPublic
AnnounceDate	2019-12-19
AnnouncementXML	Submission_2019-12-19_23:19:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ansgar Poetsch
SpeciesList	scientific name: Arbacia punctulata; NCBI TaxID: 7641;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-18 21:46:22	ID requested
⏵ 1	2019-12-19 23:19:03	announced

Dataset with its publication pending

submitter keyword: sperm chemosensation, protein copy number, membrane protein

Ansgar Poetsch
contact affiliation	Ruhr University Bochum
contact email	ansgar.poetsch@rub.de
lab head
Ansgar Poetsch
contact affiliation	Ruhr University Bochum
contact email	ansgar.poetsch@rub.de
dataset submitter

Panorama Public dataset URI