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PXD015502 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSignaling proteins of a chemosensory pathway from sperm of Arbacia punctulata
DescriptionCilia and flagella serve as cellular antennae that translate sensory information into cellular responses. In sperm, a single chemoattractant molecule triggers signaling events that evoke a Ca2+ response and chemotactic steering. Key components of the chemotactic signaling pathway from sperm of the sea urchin Arbacia punctulata were investigated. Targeted proteomics employed a spiked-in heavy isotope-labelled standard protein (QConCat) and in-gel protein digestion with trypsin. The standard protein together with the previously determined copy number of the receptor guanylate cyclase allowed determining the copy numbers for 19 polypeptides of this signaling pathway.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAnsgar Poetsch
SpeciesList scientific name: Arbacia punctulata; NCBI TaxID: 7641;
ModificationListLabel:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentTSQ Vantage
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-18 21:46:22ID requested
12019-12-19 23:19:03announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: sperm chemosensation, protein copy number, membrane protein
Contact List
Ansgar Poetsch
contact affiliationRuhr University Bochum
contact emailansgar.poetsch@rub.de
lab head
Ansgar Poetsch
contact affiliationRuhr University Bochum
contact emailansgar.poetsch@rub.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI