PXD015461-1
PXD015461 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peroxisome elevation induces stem cell differentiation and intestinal epithelial repair |
Description | To identify the regulators that directly interact with peroxisomes to promote the differentiation of intestinal stem and progenitor cells after injury, peroxisomes were isolated from both wild-type and Pex10-null flies and mass spectrometry (MS) analysis was performed. For peroxisomes isolation and mass spectrometry, 100 female 5-day-old wild-type or Pex10-null files without bleomycin treatment (BLM-0D WT, BLM-0D Pex10-null) or treatment with bleomycin for one day followed by recovery for one day (BLM-REC-1D WT, BLM-REC-1D Pex10-null) were homogenized. Each group repeat two times. The MS analysis was performed on Q ExactiveTM Mass Spectrometer (Thermo). The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Mass Spectrometer coupled online to the HPLC. Intact peptides were detected in the Orbitrap at a resolution of 70,000 and peptides were selected for MS/MS using NCE setting as 27. Protein identification was performed with MASCOT software by searching Uniprot Drosophila melanogaster. |
HostingRepository | iProX |
AnnounceDate | 2019-09-16 |
AnnouncementXML | Submission_2019-09-16_03:32:06.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gang Du |
SpeciesList | scientific name: Drosophila melanogaster; NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-09-16 03:30:21 | ID requested | |
⏵ 1 | 2019-09-16 03:32:07 | announced | |
2 | 2020-11-30 00:09:35 | announced | 2020-11-30: Update publication information |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: stem cell differentiation |
Contact List
Haiyang Chen | |
---|---|
contact affiliation | School of life science |
contact email | chenhy87@mail.sysu.edu.cn |
lab head | |
Gang Du | |
contact affiliation | School of life science |
contact email | dugang19880421@126.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |