<<< Full experiment listing

PXD015461

PXD015461 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePeroxisome elevation induces stem cell differentiation and intestinal epithelial repair
DescriptionTo identify the regulators that directly interact with peroxisomes to promote the differentiation of intestinal stem and progenitor cells after injury, peroxisomes were isolated from both wild-type and Pex10-null flies and mass spectrometry (MS) analysis was performed. For peroxisomes isolation and mass spectrometry, 100 female 5-day-old wild-type or Pex10-null files without bleomycin treatment (BLM-0D WT, BLM-0D Pex10-null) or treatment with bleomycin for one day followed by recovery for one day (BLM-REC-1D WT, BLM-REC-1D Pex10-null) were homogenized. Each group repeat two times. The MS analysis was performed on Q ExactiveTM Mass Spectrometer (Thermo). The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Mass Spectrometer coupled online to the HPLC. Intact peptides were detected in the Orbitrap at a resolution of 70,000 and peptides were selected for MS/MS using NCE setting as 27. Protein identification was performed with MASCOT software by searching Uniprot Drosophila melanogaster.
HostingRepositoryiProX
AnnounceDate2019-09-16
AnnouncementXMLSubmission_2020-11-30_00:09:34.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGang Du
SpeciesList scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-16 03:30:21ID requested
12019-09-16 03:32:07announced
22020-11-30 00:09:35announced2020-11-30: Update publication information
Publication List
Du G, Xiong L, Li X, Zhuo Z, Zhuang X, Yu Z, Wu L, Xiao D, Liu Z, Jie M, Liu X, Luo G, Guo Z, Chen H, Peroxisome Elevation Induces Stem Cell Differentiation and Intestinal Epithelial Repair. Dev Cell, 53(2):169-184.e11(2020) [pubmed]
Keyword List
submitter keyword: stem cell differentiation
Contact List
Haiyang Chen
contact affiliationSchool of life science
contact emailchenhy87@mail.sysu.edu.cn
lab head
Gang Du
contact affiliationSchool of life science
contact emaildugang19880421@126.com
dataset submitter
Full Dataset Link List
iProX dataset URI