PXD015461
PXD015461 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peroxisome elevation induces stem cell differentiation and intestinal epithelial repair |
Description | To identify the regulators that directly interact with peroxisomes to promote the differentiation of intestinal stem and progenitor cells after injury, peroxisomes were isolated from both wild-type and Pex10-null flies and mass spectrometry (MS) analysis was performed. For peroxisomes isolation and mass spectrometry, 100 female 5-day-old wild-type or Pex10-null files without bleomycin treatment (BLM-0D WT, BLM-0D Pex10-null) or treatment with bleomycin for one day followed by recovery for one day (BLM-REC-1D WT, BLM-REC-1D Pex10-null) were homogenized. Each group repeat two times. The MS analysis was performed on Q ExactiveTM Mass Spectrometer (Thermo). The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Mass Spectrometer coupled online to the HPLC. Intact peptides were detected in the Orbitrap at a resolution of 70,000 and peptides were selected for MS/MS using NCE setting as 27. Protein identification was performed with MASCOT software by searching Uniprot Drosophila melanogaster. |
HostingRepository | iProX |
AnnounceDate | 2019-09-16 |
AnnouncementXML | Submission_2020-11-30_00:09:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gang Du |
SpeciesList | scientific name: Drosophila melanogaster; NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-09-16 03:30:21 | ID requested | |
1 | 2019-09-16 03:32:07 | announced | |
⏵ 2 | 2020-11-30 00:09:35 | announced | 2020-11-30: Update publication information |
Publication List
Du G, Xiong L, Li X, Zhuo Z, Zhuang X, Yu Z, Wu L, Xiao D, Liu Z, Jie M, Liu X, Luo G, Guo Z, Chen H, Peroxisome Elevation Induces Stem Cell Differentiation and Intestinal Epithelial Repair. Dev Cell, 53(2):169-184.e11(2020) [pubmed] |
Keyword List
submitter keyword: stem cell differentiation |
Contact List
Haiyang Chen | |
---|---|
contact affiliation | School of life science |
contact email | chenhy87@mail.sysu.edu.cn |
lab head | |
Gang Du | |
contact affiliation | School of life science |
contact email | dugang19880421@126.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |