PXD015402-2

PXD015402 is an original dataset announced via ProteomeXchange.

Title	Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners
Description	Endoglin is a 180-kDa glycoprotein receptor primarily expressed by the vascular endothelium and involved in cardiovascular disease and cancer. Heterozygous mutations in the endoglin gene (ENG) cause Hereditary Hemorrhagic Telangiectasia type 1, a vascular disease that present with nose and gastrointestinal bleeding, skin and mucosa telangiectases, and arteriovenous malformations in internal organs. A circulating form of endoglin (alias soluble endoglin, sEng), proteolytically released from the membrane-bound protein, has been observed in several inflammation-related pathological conditions and appears to contribute to endothelial dysfunction and cancer development through unknown mechanisms. Membrane-bound endoglin is an auxiliary component of the TGF-β receptor complex and the extracellular region of endoglin has been shown to interact with types I and II TGF-β receptors, as well as with BMP9 and BMP10 ligands, both members of the TGF-β family. To search for novel protein interactors, we have screened a microarray containing over 9,000 unique human proteins using recombinant sEng as bait. We find that sEng binds with high affinity, at least, to 22 new proteins. Among these, we have validated the interaction of endoglin with galectin-3, a secreted member of the lectin family with capacity to bind membrane glycoproteins, and with tripartite motif-containing protein 21 (TRIM21), an E3 ubiquitin-protein ligase. Using human endothelial cells and Chinese hamster ovary cells, we showed that endoglin co-immunoprecipitates and co-localizes with galectin-3 or TRIM21. These results open new research avenues on endoglin function and regulation.
HostingRepository	PRIDE
AnnounceDate	2019-09-25
AnnouncementXML	Submission_2019-09-26_03:23:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Casado
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-11 01:14:50	ID requested
1	2019-09-23 04:53:19	announced
⏵ 2	2019-09-26 03:23:56	announced	2019-09-26: Updated contact info

Gallardo-Vara E, Ruiz-Llorente L, Casado-Vela J, Ruiz-Rodr, í, guez MJ, L, ó, pez-Andr, é, s N, Pattnaik AK, Quintanilla M, Bernabeu C, Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners. Cells, 8(9):(2019) [pubmed]

submitter keyword: endothelial cells

endoglin

TGF-β

BMP

HHT

cancer

preeclampsia

TRIM family

galectin family

protein-protein interactions

Miguel Quintanilla
contact affiliation	Centro de Investigaciones Biolgicas, Consejo Superior de Investigaciones Cientficas (CSIC), and Centro de Investigacin Biomedica en Red de Enfermedades Raras (CIBERER), Madrid 28040, Spain; eunate.gallardo@yale.edu (E.G.-V.); lruiz.llorente@cib.csic.es (L.R.-L.); bernabeu.c@cib.csic.es (C.B.)
contact email	bernabeu.c@cib.csic.es
lab head
Juan Casado
contact affiliation	UC3M
contact email	jucasado@ing.uc3m.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD015402

PRIDE project URI

• PRIDE
  1. PXD015402
     1. Label: PRIDE project
     2. Name: Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners