PXD015394-1

PXD015394 is an original dataset announced via ProteomeXchange.

Title	MED12 wraps around CDK8 and utilizes an activation helix to stimulate its kinase activity
Description	Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 8 (CDK8), cyclin-C, and subunit 12 of the Mediator complex (MED12). Cross-linking was performed using different cross-linking chemistries: (1) disuccinimidyl suberate (DSS); (2) a combination of pimelic acid dihydrazide (PDH) and the coupling reagent, DMTMM.
HostingRepository	PRIDE
AnnounceDate	2020-01-16
AnnouncementXML	Submission_2020-01-16_07:34:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Leitner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-10 06:41:46	ID requested
⏵ 1	2020-01-16 07:34:38	announced
2	2020-02-26 03:28:28	announced	2020-02-26: Updated publication reference for PubMed record(s): 31988137. 2020-02-26: Updated publication reference for DOI(s): 10.1038/s41591-019-0738-2.
3	2023-11-14 08:50:41	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Chemical cross-linking

DSS

PDH

DMTMM

structural proteomics

mediator complex

kinase

Claus-D. Kuhn
contact affiliation	University of Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany
contact email	claus.kuhn@uni-bayreuth.de
lab head
Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015394

PRIDE project URI

• PRIDE
  1. PXD015394
     1. Label: PRIDE project
     2. Name: MED12 wraps around CDK8 and utilizes an activation helix to stimulate its kinase activity