PXD015394 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A precisely positioned MED12 activation helix stimulates CDK8 kinase activity |
Description | Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 8 (CDK8), cyclin-C, and subunit 12 of the Mediator complex (MED12). Cross-linking was performed using different cross-linking chemistries: (1) disuccinimidyl suberate (DSS); (2) a combination of pimelic acid dihydrazide (PDH) and the coupling reagent, DMTMM. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:50:40.596.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexander Leitner |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-10 06:41:46 | ID requested | |
1 | 2020-01-16 07:34:38 | announced | |
2 | 2020-02-26 03:28:28 | announced | 2020-02-26: Updated publication reference for PubMed record(s): 31988137.
2020-02-26: Updated publication reference for DOI(s): 10.1038/s41591-019-0738-2. |
⏵ 3 | 2023-11-14 08:50:41 | announced | 2023-11-14: Updated project metadata. |
Publication List
Klatt F, Leitner A, Kim IV, Ho-Xuan H, Schneider EV, Langhammer F, Weinmann R, M, ü, ller MR, Huber R, Meister G, Kuhn CD, A precisely positioned MED12 activation helix stimulates CDK8 kinase activity. Proc Natl Acad Sci U S A, 117(6):2894-2905(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Chemical cross-linking |
DSS |
PDH |
DMTMM |
structural proteomics |
mediator complex |
kinase |
Contact List
Claus-D. Kuhn |
contact affiliation | University of Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany |
contact email | claus.kuhn@uni-bayreuth.de |
lab head | |
Alexander Leitner |
contact affiliation | ETH Zurich, Institute of Molecular Systems Biology |
contact email | leitner@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015394
- Label: PRIDE project
- Name: A precisely positioned MED12 activation helix stimulates CDK8 kinase activity