PXD015380-1

PXD015380 is an original dataset announced via ProteomeXchange.

Title	Multi-omic analysis of gametogenesis reveals a novel signature at the promoters and distal enhancers of active genes
Description	Epigenetic regulation of gene expression is tightly controlled by the dynamic modification of histones by chemical groups, the diversity of which has largely expanded over the past decade with the discovery of lysine acylations, catalyzed from acyl-coenzymes A. Here, we investigated the dynamics of lysine acetylation and crotonylation on histones H3 and H4 during mouse spermatogenesis. Lysine crotonylation appeared to be of significant abundance compared to acetylation, particularly on Lys27 of histone H3 (H3K27cr) that accumulates in sperm in a cleaved form of H3. We identified the genomic localization of H3K27cr and studied its effects on transcription compared to the classical active mark H3K27ac at promoters and distal enhancers. The presence of both marks was strongly associated with highest gene expression. Assessment of their co-localization with transcription regulators (SLY, SOX30) and chromatin-binding proteins (BRD4, BORIS and CTCF) indicated systematic highest binding when both active marks were present and different selective binding when present alone at chromatin. H3K27cr and H3K27ac finally mark the building of some sperm super-enhancers. This integrated analysis of omics data provides an unprecedented level of understanding of gene expression regulation by H3K27cr in comparison to H3K27ac, and reveals both synergistic and specific actions of each histone modification.
HostingRepository	PRIDE
AnnounceDate	2020-03-26
AnnouncementXML	Submission_2020-03-26_01:54:10.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015380
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Delphine Pflieger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	Phospho; Methyl; Crotonyl; Dimethyl; Acetyl; Carbamidomethyl; Trimethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-09 07:43:25	ID requested
⏵ 1	2020-03-26 01:59:24	announced
2	2020-07-08 06:40:41	announced	2020-07-08: Updated publication reference for PubMed record(s): 32182340.

Crespo M, Damont A, Blanco M, Lastrucci E, Kennani SE, Ialy-Radio C, Khattabi LE, Terrier S, Louwagie M, Kieffer-Jaquinod S, Hesse AM, Bruley C, Chantalat S, Govin J, Fenaille F, Battail C, Cocquet J, Pflieger D, Multi-omic analysis of gametogenesis reveals a novel signature at the promoters and distal enhancers of active genes. Nucleic Acids Res, 48(8):4115-4138(2020) [pubmed]

submitter keyword: Mouse spermatogenesis, Histones, Crotonylation, Promoters and enhancers, Omics data integration

Virginie Brun
contact affiliation	EDyP/BGE/IRIG/CEA Grenoble
contact email	virginie.brun@cea.fr
lab head
Delphine Pflieger
contact affiliation	CNRS
contact email	delphine.pflieger@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015380
     1. Label: PRIDE project
     2. Name: Multi-omic analysis of gametogenesis reveals a novel signature at the promoters and distal enhancers of active genes