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PXD015258-1

PXD015258 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTMT10 controlled mixtures - SILAC HeLa UPS1, acquired using SPS-MS3
DescriptionThe controlled mixtures were prepared to evaluate the ability of our proposed approach when dealing with the complicated design, e.g. multiple TMT mixtures with technical replicates. 500, 333, 250, and 62.5 fmol UPS1 peptides were spiked-into 50 g SILAC HeLa peptides in duplicate. It produced a dilution series corresponding to 1, 0.667, 0.5, and 0.125 of the highest UPS1 peptide amount (500 fmol). In addition, a reference sample was generated by pooling all four diluted UPS1 peptide samples (286.5 fmol) and combined with 50 g of SILAC HeLa in duplicate. These 10 replicates were labeled with TMT10-plex reagents and mixed together to pass LC-MS/MS analysis. The procedure was repeated to generate a total of five such controlled mixtures. To assess technical variability, three technical replicates were prepared for each mixture. Totally there are 15 MS runs from 5 TMT mixtures. LC-MS/MS was performed using an EASY-nLC 1200 ultrahigh pressure liquid chromatography (UHPLC) connected to an Orbitrap Fusion Lumos Tribrid and equipped with an EASY-spray source (Thermo Fisher Scientific, San Jose, CA). The data was acquired using an MS2/MS3 (also called multinotch MS3 or Synchronous Precursor Selection, SPS).
HostingRepositoryMassIVE
AnnounceDate2019-09-03
AnnouncementXMLSubmission_2019-09-03_08:18:21.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMeena Choi
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-30 15:46:21ID requested
12019-09-03 08:18:21announced
Publication List
no publication
Keyword List
submitter keyword: TMT10plex, Controlled Mixture, Lumos, Proteomics
Contact List
Tom Dunkley
contact affiliationRoche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, Hoffmann-La Roche Ltd
contact emailtom.dunkley@roche.com
lab head
Olga Vitek
contact affiliationKhoury College of Computer Sciences, Northeastern University
contact emailo.vitek@northeastern.edu
lab head
Meena Choi
contact affiliationNortheastern University
contact emailmnchoi67@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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