PXD015214 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Absolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation |
Description | Gene transcription in eukaryotes is regulated through dynamic interactions of a variety of different proteins with DNA in the context of chromatin. Here, we used mass spectrometry for absolute quantification of the nuclear proteome during two stages of Drosophila embryogenesis. Before proteomics analysis, we counted the absolute amount of genomic copies and used a standard range of proteins (UPS2 standard) for absolute quantification of protein copy numbers per 1C genome. These analyses provide comprehensive information about the absolute copy number of several thousand proteins and reveal unexpected relationships between the abundance of histone-modifying and -binding proteins and the chromatin landscape that they generate and interact with. These data provide a foundation towards a quantitative understanding of gene regulation in Drosophila. |
HostingRepository | PRIDE |
AnnounceDate | 2023-02-01 |
AnnouncementXML | Submission_2023-02-01_11:34:04.065.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | RikLindeboom |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-29 05:32:00 | ID requested | |
⏵ 1 | 2023-02-01 11:34:04 | announced | |
2 | 2023-11-14 08:34:12 | announced | 2023-11-14: Updated project metadata. |
Publication List
Bonnet J, Lindeboom RGH, Pokrovsky D, Stricker G, Ç, elik MH, Rupp RAW, Gagneur J, Vermeulen M, Imhof A, M, ü, ller J, Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation. Dev Cell, 51(5):632-644.e6(2019) [pubmed] |
Keyword List
submitter keyword: absolute proteome quantification/ histone modification quantification, PRC2, H3K27 methylation, Drosophila |
Contact List
JürgMüller |
contact affiliation | Max-Planck Institute of Biochemistry, Laboratory of Chromatin Biology, Martinsried, Germany |
contact email | muellerj@biochem.mpg.de |
lab head | |
RikLindeboom |
contact affiliation | Radboud Institute for Molecular Life Sciences |
contact email | r.lindeboom@science.ru.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015214
- Label: PRIDE project
- Name: Absolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation