PXD015214

PXD015214 is an original dataset announced via ProteomeXchange.

Title	Absolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation
Description	Gene transcription in eukaryotes is regulated through dynamic interactions of a variety of different proteins with DNA in the context of chromatin. Here, we used mass spectrometry for absolute quantification of the nuclear proteome during two stages of Drosophila embryogenesis. Before proteomics analysis, we counted the absolute amount of genomic copies and used a standard range of proteins (UPS2 standard) for absolute quantification of protein copy numbers per 1C genome. These analyses provide comprehensive information about the absolute copy number of several thousand proteins and reveal unexpected relationships between the abundance of histone-modifying and -binding proteins and the chromatin landscape that they generate and interact with. These data provide a foundation towards a quantitative understanding of gene regulation in Drosophila.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:34:12.062.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rik Lindeboom
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-29 05:32:00	ID requested
1	2023-02-01 11:34:04	announced
⏵ 2	2023-11-14 08:34:12	announced	2023-11-14: Updated project metadata.

Bonnet J, Lindeboom RGH, Pokrovsky D, Stricker G, Ç, elik MH, Rupp RAW, Gagneur J, Vermeulen M, Imhof A, M, ü, ller J, Quantification of Proteins and Histone Marks in Drosophila Embryos Reveals Stoichiometric Relationships Impacting Chromatin Regulation. Dev Cell, 51(5):632-644.e6(2019) [pubmed]

submitter keyword: absolute proteome quantification/ histone modification quantification, PRC2, H3K27 methylation, Drosophila

Jürg Müller
contact affiliation	Max-Planck Institute of Biochemistry, Laboratory of Chromatin Biology, Martinsried, Germany
contact email	muellerj@biochem.mpg.de
lab head
Rik Lindeboom
contact affiliation	Radboud Institute for Molecular Life Sciences
contact email	r.lindeboom@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD015214

PRIDE project URI

• PRIDE
  1. PXD015214
     1. Label: PRIDE project
     2. Name: Absolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation