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PXD015150-1

PXD015150 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInduced Systemic Effects of Engineering the Cell Membrane Composition in Bacillus subtilis
DescriptionEngineering bacterial cells to contain defined fatty acid compositions within their cell membranes establishes an in vivo experimental platform for membrane biophysics. Yet before fully realizing the potential of this system, it is prudent to understand the systemic changes induced by the labeling procedure itself. In this work, the systemic induced changes in cell are studied via analysis of cellular membrane compositions and shotgun proteomic analysis to compare expression levels of selected cellular proteins; in response to the labeling of Bacillus subtilis using cerulenin to inhibit fatty acid synthesis and subsequently providing selected fatty acids exogenously to be incorporated by the cell. Changes in the expression of enzymes involved in fatty acid biosynthesis and degradation are observed and consistent with expectations. Key findings from this analysis include an alteration in lipid headgroup distribution, with an increase in phophatidylglycerol lipids and decrease in phosphatidylethanolamine lipids, possibly providing a fluidizing effect on the cell membrane in response to the induced change in membrane composition. Beyond this, changes to cell wall enzymes and isoprenoid lipid production are also seen, which may influence membrane organization, and indeed the well-known lipid raft-associated protein flotillin was found to be substantially down regulated in the labeled cells, as was the actin-like protein mreB. Taken as a whole, this study provides a greater depth of understanding for this important cell membrane experimental platform and presents a number of new connections to be explored between an enforced cell membrane fatty acid composition and lipid headgroup and raft cytoskeletal associated proteins.
HostingRepositoryMassIVE
AnnounceDate2020-05-01
AnnouncementXMLSubmission_2020-05-01_10:13:02.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSuresh Poudel
SpeciesList scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationListiodoacetamide - site C; L-methionine sulfoxide
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-23 11:34:24ID requested
12020-05-01 10:13:03announced
Publication List
no publication
Keyword List
submitter keyword: Bacillus subtilis, lipidomics, proteomics, fatty acid, membrane
Contact List
Robert L. Hettich
contact affiliationOak Ridge National Laboratory
contact emailhettichrl@ornl.gov
lab head
Suresh Poudel
contact affiliationSt Jude Childrens Research Hospital
contact emailspoudel1@stjude.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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