PXD015059 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome and phosphoproteome response of the diatom Phaeodactylum tricornutum under varying nitrogen and iron |
Description | The photosynthetic model marine diatom Phaeodactylum tricornutum was cultured under conditions varying nitrogen content and availability, or varying iron concentrations over diel cycles to elicit shifts in the proteome and phosphoproteome. For the nitrogen experiments, cells were grown to mid-exponential phase on ammonium (880 uM), collected by centrifugation and resuspended in N-free media for 2 hrs, and spiked with nitrate (150 uM) for 90 min. Cells were again collected by centrifugation, washed in N-free media, and resuspended in experimental treatments with no nitrogen (N-) and a nitrogen source (300 uM) provided as ammonium, nitrite, or nitrate. Each experimental treatment was sampled after 15 min, 45 min, and 18 hr. For iron experiments, steady-state axenic cultures were maintained under a diel cycle of 12h light: 12h darkness and at three different concentrations of Fe' (Fe' is the sum of all Fe species not complexed to EDTA): a low concentration (20 pM Fe'), intermediate concentration (40 pM Fe') and at Fe-replete levels (400 pM Fe'). Samples were taken every four hours. |
HostingRepository | PRIDE |
AnnounceDate | 2020-04-17 |
AnnouncementXML | Submission_2020-04-16_22:16:52.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD015059 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Matthew Monroe |
SpeciesList | scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850; |
ModificationList | Phospho; Oxidation; iTRAQ8plex; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-16 05:34:17 | ID requested | |
⏵ 1 | 2020-04-16 22:16:53 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: iron, diel, diatom |
Contact List
Andrew E. Allen |
contact affiliation | Scripps Institution of Oceanography, University of California |
contact email | aallen@ucsd.edu |
lab head | |
Matthew Monroe |
contact affiliation | Pacific Northwest National Laboratory |
contact email | matthew.monroe@pnnl.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD015059 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015059
- Label: PRIDE project
- Name: Proteome and phosphoproteome response of the diatom Phaeodactylum tricornutum under varying nitrogen and iron