PXD015059

PXD015059 is an original dataset announced via ProteomeXchange.

Title	Proteome and phosphoproteome response of the diatom Phaeodactylum tricornutum under varying nitrogen and iron
Description	The photosynthetic model marine diatom Phaeodactylum tricornutum was cultured under conditions varying nitrogen content and availability, or varying iron concentrations over diel cycles to elicit shifts in the proteome and phosphoproteome. For the nitrogen experiments, cells were grown to mid-exponential phase on ammonium (880 uM), collected by centrifugation and resuspended in N-free media for 2 hrs, and spiked with nitrate (150 uM) for 90 min. Cells were again collected by centrifugation, washed in N-free media, and resuspended in experimental treatments with no nitrogen (N-) and a nitrogen source (300 uM) provided as ammonium, nitrite, or nitrate. Each experimental treatment was sampled after 15 min, 45 min, and 18 hr. For iron experiments, steady-state axenic cultures were maintained under a diel cycle of 12h light: 12h darkness and at three different concentrations of Fe' (Fe' is the sum of all Fe species not complexed to EDTA): a low concentration (20 pM Fe'), intermediate concentration (40 pM Fe') and at Fe-replete levels (400 pM Fe'). Samples were taken every four hours.
HostingRepository	PRIDE
AnnounceDate	2020-04-17
AnnouncementXML	Submission_2020-04-16_22:16:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015059
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationList	Phospho; Oxidation; iTRAQ8plex; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-16 05:34:17	ID requested
⏵ 1	2020-04-16 22:16:53	announced

Dataset with its publication pending

submitter keyword: iron, diel, diatom

Andrew E. Allen
contact affiliation	Scripps Institution of Oceanography, University of California
contact email	aallen@ucsd.edu
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015059
     1. Label: PRIDE project
     2. Name: Proteome and phosphoproteome response of the diatom Phaeodactylum tricornutum under varying nitrogen and iron