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PXD015018 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntroducing plasma/serum glycodepletion for the targeted proteomics analysis of cytolysis biomarkers
DescriptionA major class of clinical biomarkers is constituted of intracellular proteins which are leaking into the blood following ischemia, exposure to toxic xenobiotics or mechanical aggression. Their ectopic presence in plasma/serum is an indicator of tissue damage and raises a warning signal. These proteins, referred to as cytolysis biomarkers, are generally of cytoplasmic origin and as such, are devoid of glycosylation. In contrast, most plasma/serum proteins originate from the hepatic secretory pathway and are heavily glycosylated (at the exception of albumin). Recent advances in targeted proteomics have supported the parallelized evaluation of new blood biomarkers. However, these analytical methods must be combined with prefractionation strategies that reduce the complexity of plasma/serum matrix. In this article, we present the glycodepletion method, which reverses the hydrazide-based glycocapture concept to remove plasma/serum glycoproteins from plasma/serum matrix and facilitates the detection of cytolysis biomarkers. Glycodepletion was integrated to a targeted proteomics pipeline to evaluate 4 liver cytolysis biomarker candidates in the context of acetaminophen-induced acute hepatitis.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterVirginie Brun
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine
InstrumentQTRAP 6500
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-13 15:18:07ID requested
12019-08-13 15:22:47announced
Publication List
Maes P, Donadio-Andréi S, Louwagie M, Couté Y, Picard G, Lacoste C, Bruley C, Garin J, Ichai P, Faivre J, Jaquinod M, Brun V, Introducing plasma/serum glycodepletion for the targeted proteomics analysis of cytolysis biomarkers. Talanta, 170():473-480(2017) [pubmed]
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted
Contact List
Virginie Brun
contact affiliationCEA
contact emailvirginie.brun@cea.fr
dataset submitter
Virginie Brun
contact affiliationCEA
contact emailvirginie.brun@cea.fr
lab head
Full Dataset Link List
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