PXD015017-1

PXD015017 is an original dataset announced via ProteomeXchange.

Title	Dynamics of protein expression of estrogen receptor alpha signalling in MCF-7 breast cancer cells
Description	Estrogen receptor alpha (ERα)-mediated proliferation of breast cancer cells is facilitated through expression of multiple primary target genes, products of which induce a secondary response to stimulation. To differentiate between the primary and secondary target proteins of ERα signaling, we measured dynamics of protein expression induced by 17β-estradiol in MCF-7 breast cancer cells. Measurement of the global proteomic effects of estradiol by stable isotope labeling by amino acids in cell culture resulted in identification of 103 estrogen-regulated proteins, with only 40 of the corresponding genes having estrogen response elements. Selected reaction monitoring assays were used to validate the differential expression of 19 proteins and measure the dynamics of their expression within 72 hours after estradiol stimulation, and in the absence or presence of 4-hydroxytamoxifen, to confirm ERα-mediated signaling. Dynamics of protein expression unambiguously revealed early and delayed response proteins and well correlated with presence or absence of estrogen response elements in the corresponding genes.. Finally, we quantified dynamics of protein expression in a rarely studied network of transcription factors with a negative feedback loop (ERα-EGR3-NAB2). Since NAB2 protein is a repressor of EGR3-induced transcription, siRNA-mediated silencing of NAB2 resulted in the enhanced expression of the EGR3-induced protein ITGA2. To conclude, we provided a high-quality proteomic resource to supplement genomic and transcriptomic studies of ERα signaling.
HostingRepository	PeptideAtlas
AnnounceDate	2019-08-13
AnnouncementXML	Submission_2019-08-13_15:20:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrei Drabovich
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-13 15:16:53	ID requested
⏵ 1	2019-08-13 15:20:21	announced

Drabovich AP, Pavlou MP, Schiza C, Diamandis EP, Dynamics of Protein Expression Reveals Primary Targets and Secondary Messengers of Estrogen Receptor Alpha Signaling in MCF-7 Breast Cancer Cells. Mol Cell Proteomics, 15(6):2093-107(2016) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

Andrei Drabovich
contact affiliation	University of Toronto
contact email	andrei.drabovich@utoronto.ca
dataset submitter
Andrei Drabovich
contact affiliation	Department of Laboratory Medicine and Pathobiology, University of Toronto
contact email	andrei.drabovich@utoronto.ca
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI