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PXD015017

PXD015017 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamics of protein expression of estrogen receptor alpha signalling in MCF-7 breast cancer cells
DescriptionEstrogen receptor alpha (ERα)-mediated proliferation of breast cancer cells is facilitated through expression of multiple primary target genes, products of which induce a secondary response to stimulation. To differentiate between the primary and secondary target proteins of ERα signaling, we measured dynamics of protein expression induced by 17β-estradiol in MCF-7 breast cancer cells. Measurement of the global proteomic effects of estradiol by stable isotope labeling by amino acids in cell culture resulted in identification of 103 estrogen-regulated proteins, with only 40 of the corresponding genes having estrogen response elements. Selected reaction monitoring assays were used to validate the differential expression of 19 proteins and measure the dynamics of their expression within 72 hours after estradiol stimulation, and in the absence or presence of 4-hydroxytamoxifen, to confirm ERα-mediated signaling. Dynamics of protein expression unambiguously revealed early and delayed response proteins and well correlated with presence or absence of estrogen response elements in the corresponding genes.. Finally, we quantified dynamics of protein expression in a rarely studied network of transcription factors with a negative feedback loop (ERα-EGR3-NAB2). Since NAB2 protein is a repressor of EGR3-induced transcription, siRNA-mediated silencing of NAB2 resulted in the enhanced expression of the EGR3-induced protein ITGA2. To conclude, we provided a high-quality proteomic resource to supplement genomic and transcriptomic studies of ERα signaling.
HostingRepositoryPeptideAtlas
AnnounceDate2019-08-13
AnnouncementXMLSubmission_2019-08-13_15:20:20.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrei Drabovich
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine
InstrumentTSQ Vantage
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-13 15:16:53ID requested
12019-08-13 15:20:21announced
Publication List
Drabovich AP, Pavlou MP, Schiza C, Diamandis EP, Dynamics of Protein Expression Reveals Primary Targets and Secondary Messengers of Estrogen Receptor Alpha Signaling in MCF-7 Breast Cancer Cells. Mol Cell Proteomics, 15(6):2093-107(2016) [pubmed]
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted
Contact List
Andrei Drabovich
contact affiliationUniversity of Toronto
contact emailandrei.drabovich@utoronto.ca
dataset submitter
Andrei Drabovich
contact affiliationDepartment of Laboratory Medicine and Pathobiology, University of Toronto
contact emailandrei.drabovich@utoronto.ca
lab head
Full Dataset Link List
PeptideAtlas dataset URI
PASSEL experiment URI
PASSEL transition group browser URI