PXD015001-1

PXD015001 is an original dataset announced via ProteomeXchange.

Title	HDL proteome remodeling post-stroke
Description	Prospective cohort studies and meta-analyses examining the relationship between HDL-cholesterol (C) and stroke risk are discordant and question the value of HDL-C as a marker for stroke risk prediction. However, the relationship between other metrics of HDL, function and proteome, and stroke remain unexplored. We investigated changes in HDL protein composition and function -cholesterol efflux capacity (CEC)- after acute ischemic stroke, and their relationship to long-term functional and neuronal recovery after stroke. Plasma samples were collected from healthy controls (N = 35) and from stroke patients either 24 (early, N = 35) or 96 hours (late, N = 20) after stroke onset. ABCA1 mediated CEC was measured using murine macrophages. Stroke recovery was assessed at 3 months after stroke event using the Modified Rankin Scores (MRS) and the NIH Stroke Scale (NIHSS). Proteomic analysis of HDL by parallel reaction monitoring indicated a distinct time-dependent remodeling after stroke. 15 proteins were significantly altered following stroke, with 6 proteins significantly changing between 24 and 96 h post stroke. Inflammation-related proteins (SAA1, SAA2, PON1) were increased at both time points. Macrophage CEC, consistent with inflammatory remodeling of HDL proteome, was reduced by 50% (P<0.0001) in both early and late post-stroke samples compared to the controls. Changes in 6 post-stroke significantly correlated with stroke recovery scores (P<0.05). Further, the multiple linear regression model adjusting for baseline stroke severity confirmed that these proteins that predict the stroke recovery. Changes in HDL associated proteins within the first 96 hours post stroke could be used as markers to predict functional stroke recovery.
HostingRepository	PanoramaPublic
AnnounceDate	2021-01-24
AnnouncementXML	Submission_2021-01-24_17:21:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Deanna Plubell
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:15N(1); Label:15N(2); Label:15N(3); Label:15N(4)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-12 21:41:19	ID requested
⏵ 1	2021-01-24 17:21:29	announced

Plubell DL, Fenton AM, Rosario S, Bergstrom P, Wilmarth PA, Clark WM, Zakai NA, Quinn JF, Minnier J, Alkayed NJ, Fazio S, Pamir N, High-Density Lipoprotein Carries Markers That Track With Recovery From Stroke. Circ Res, 127(10):1274-1287(2020) [pubmed]

submitter keyword: parallel reaction monitoring, HDL, stroke

Nathalie Pamir
contact affiliation	OHSU
contact email	pamir@ohsu.edu
lab head
Deanna Plubell
contact affiliation	OHSU
contact email	plubell@uw.edu
dataset submitter

Panorama Public dataset URI