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PXD014976 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes
DescriptionArctigenin (ATG) is a major component of Fructus Arctii, which as a traditional herbal remedy reduced proteinuria in diabetic patients. However, whether ATG specifically provided renoprotection in DKD was not known. Here we report that ATG administration is sufficient to attenuate proteinuria and podocyte injury in mouse models of diabetes. Transcriptomic analysis of diabetic mouse glomeruli showed that cell adhesion and inflammation are two key pathways affected by ATG treatment, and mass spectrometry analysis identified protein phosphatase 2A (PP2A) as one of the top ATG-interacting proteins in renal cells. Enhanced PP2A activity by ATG reduces p65 NF-κB-mediated inflammatory response and high glucose-induced migration in cultured podocytes via its interaction with Drebrin-1. Importantly, podocyte-specific Pp2a deletion in mice exacerbates DKD injury and abrogates the ATG-mediated renoprotection. Collectively, our results clearly demonstrate a renoprotective mechanism of ATG via PP2A activation and establish PP2A as a potential target for DKD progression.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTong Liu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListPhospho; Oxidation; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-12 00:57:35ID requested
12019-11-08 09:07:06announced
Publication List
Zhong Y, Lee K, Deng Y, Ma Y, Chen Y, Li X, Wei C, Yang S, Wang T, Wong NJ, Muwonge AN, Azeloglu EU, Zhang W, Das B, He JC, Liu R, Arctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes. Nat Commun, 10(1):4523(2019) [pubmed]
Keyword List
submitter keyword: Diabetic kidney disease, Arctigenin, podocytes, PP2A, actin cytoskeleton, Drebrin-1
Contact List
John Cijiang He
contact affiliationJohn Cijiang He, MD/PhD Division of Nephrology, Box 1243 Mount Sinai School of Medicine One Gustave L Levy Place, New York NY 10029 Tel: 212-659-1703, Fax: 212-987-0389
contact emailcijiang.he@mssm.edu
lab head
Tong Liu
contact affiliationRutgers University
contact emaillinto@njms.rutgers.edu
dataset submitter
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