PXD014894 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of NK cell-derived extracellular vesicles. |
Description | Natural killer (NK) cells contribute to immunosurveillance and first-line defense in the control of tumor growth and metastasis diffusion. NKEVs are constitutively secreted, are biologically active, reflect the protein and genetic repertoire of their originating cells and exert anti-tumor activity in vitro and in vivo. NKEVs from tumor-conditioned NK cells interact with naïve NK cells promoting their cytotoxic activity. In cancer NK cells exhibit profound defects in degranulation ability, a status probably reflected by their NKEVs. Hence, NKEVs could contribute to improve cancer therapy by interacting with tumor and/or immune cells at the same time sensing the actual NK cell status in cancer patients. Here we investigated the role of NKEVs in stimulating the immune system and developed an immune enzymatic test (NKExoELISA) to sense the systemic NK cell status by measuring plasma NK-derived exosomes through combined capture of exosomes, expressing typical EV (tsg101) and NK cell (CD56) markers. We analyzed by LC-MS/MS the protein content from NKEVs evaluating proteins differentially expressed in exosomes (NKExo), vescicles (NKMV) and total cell extract (Tot extr) from parental NK cells. Proteomic data confirmed the presence of many EV markers and detected several proteins involved in immune response, cell adhesion and complement biological processes. |
HostingRepository | PRIDE |
AnnounceDate | 2020-04-03 |
AnnouncementXML | Submission_2020-04-02_23:06:01.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Serena Camerini |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-02 06:40:56 | ID requested | |
⏵ 1 | 2020-04-02 23:06:03 | announced | |
Publication List
Federici C, Shahaj E, Cecchetti S, Camerini S, Casella M, Iessi E, Camisaschi C, Paolino G, Calvieri S, Ferro S, Cova A, Squarcina P, Bertuccini L, Iosi F, Huber V, Lugini L, Natural-Killer-Derived Extracellular Vesicles: Immune Sensors and Interactors. Front Immunol, 11():262(2020) [pubmed] |
Keyword List
submitter keyword: proteomics natural killer cells exosomes microvesicles |
Contact List
serena camerini |
contact affiliation | Core facilities National Institute of Health Viale Regina Elena 299 00161 Rome Italy |
contact email | serena.camerini@iss.it |
lab head | |
Serena Camerini |
contact affiliation | Istituto superiore di sanità |
contact email | serena.camerini@iss.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014894
- Label: PRIDE project
- Name: Proteomic analysis of NK cell-derived extracellular vesicles.