PXD014894

PXD014894 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of NK cell-derived extracellular vesicles.
Description	Natural killer (NK) cells contribute to immunosurveillance and first-line defense in the control of tumor growth and metastasis diffusion. NKEVs are constitutively secreted, are biologically active, reflect the protein and genetic repertoire of their originating cells and exert anti-tumor activity in vitro and in vivo. NKEVs from tumor-conditioned NK cells interact with naïve NK cells promoting their cytotoxic activity. In cancer NK cells exhibit profound defects in degranulation ability, a status probably reflected by their NKEVs. Hence, NKEVs could contribute to improve cancer therapy by interacting with tumor and/or immune cells at the same time sensing the actual NK cell status in cancer patients. Here we investigated the role of NKEVs in stimulating the immune system and developed an immune enzymatic test (NKExoELISA) to sense the systemic NK cell status by measuring plasma NK-derived exosomes through combined capture of exosomes, expressing typical EV (tsg101) and NK cell (CD56) markers. We analyzed by LC-MS/MS the protein content from NKEVs evaluating proteins differentially expressed in exosomes (NKExo), vescicles (NKMV) and total cell extract (Tot extr) from parental NK cells. Proteomic data confirmed the presence of many EV markers and detected several proteins involved in immune response, cell adhesion and complement biological processes.
HostingRepository	PRIDE
AnnounceDate	2020-04-03
AnnouncementXML	Submission_2020-04-02_23:06:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Serena Camerini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-02 06:40:56	ID requested
⏵ 1	2020-04-02 23:06:03	announced

Federici C, Shahaj E, Cecchetti S, Camerini S, Casella M, Iessi E, Camisaschi C, Paolino G, Calvieri S, Ferro S, Cova A, Squarcina P, Bertuccini L, Iosi F, Huber V, Lugini L, Natural-Killer-Derived Extracellular Vesicles: Immune Sensors and Interactors. Front Immunol, 11():262(2020) [pubmed]

submitter keyword: proteomics natural killer cells exosomes microvesicles

serena camerini
contact affiliation	Core facilities National Institute of Health Viale Regina Elena 299 00161 Rome Italy
contact email	serena.camerini@iss.it
lab head
Serena Camerini
contact affiliation	Istituto superiore di sanità
contact email	serena.camerini@iss.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD014894

PRIDE project URI

• PRIDE
  1. PXD014894
     1. Label: PRIDE project
     2. Name: Proteomic analysis of NK cell-derived extracellular vesicles.