PXD014806

PXD014806 is an original dataset announced via ProteomeXchange.

Title	Comparison of Protein Expression During Early Phases of Limb Regeneration Between Regeneration-permissive and Regeneration-deficient Axolotl
Description	In this project, we studied the proteomic profiles of the early stages of blastema formation of neotenic and metamorphic axolotls after limb amputation by means of LC-MS/MS technology. We quantified a total of 714 proteins having an adjusted p < 0.01 with FC greater or equal to 2 between two conditions. Principal component analysis revealed a conspicuous clustering between neotenic and metamorphic samples at 7 days post-amputation. Different set of proteins was identified as differentially expressed at all of the time points (1, 4, and 7 days post-amputations against day0) for neotenic and metamorphic stages. Although functional enrichment analyses underline the presence of common pathways between regenerative and non-regenerative stages, cell proliferation and its regulation associated pathways, immune system related pathways and muscle tissue and ECM remodeling and degradation pathways were represented at different rate between both stages.
HostingRepository	PRIDE
AnnounceDate	2019-11-25
AnnouncementXML	Submission_2020-01-14_07:32:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014806
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Baris Suzek
SpeciesList	scientific name: Ambystoma mexicanum; NCBI TaxID: 8296;
ModificationList	Ammonia-loss; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	SYNAPT G2-Si; ACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-29 05:37:39	ID requested
1	2019-11-25 07:20:26	announced
⏵ 2	2020-01-14 07:32:35	announced	2020-01-14: Updated publication reference for PubMed record(s): 31767146.

Sibai M, Altunta, ş E, S, ü, zek BE, Ş, ahin B, Parlayan C, Ö, zt, ü, rk G, Baykal AT, Demircan T, Comparison of protein expression profile of limb regeneration between neotenic and metamorphic axolotl. Biochem Biophys Res Commun, 522(2):428-434(2020) [pubmed]

submitter keyword: Proteomics

Axolotl

Limb regeneration

Metamorphosis

Gene expression profile

Turan Demircan
contact affiliation	Regenerative and Restorative Medicine Research Center, REMER, Istanbul Medipol University, Istanbul, Turkey Department of Medical Biology, School of Medicine, Mugla Sitki Kocman University, Mugla, Turkey
contact email	turandemircan@gmail.com
lab head
Baris Suzek
contact affiliation	Mugla Sitki Kocman University
contact email	barissuzek@mu.edu.tr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014806

PRIDE project URI

• PRIDE
  1. PXD014806
     1. Label: PRIDE project
     2. Name: Comparison of Protein Expression During Early Phases of Limb Regeneration Between Regeneration-permissive and Regeneration-deficient Axolotl