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PXD014738 is an original dataset announced via ProteomeXchange.

Dataset Summary

  • Title: BS3 crosslinking of GRC3 and Las1
  • Description: Ribosome assembly is a complex process involving the coordination of multiple enzymes. A recently discovered endoribonuclease (RNase), Las1, and the poly-nucleotide kinase (PNK), Grc3, assemble into a complex. To attempt to understand the coordination of the two enzymes in this complex, we performed chemical crosslinking and mass spectrometry and also solved a series of cryo-electron microscopy structures of RNase PNK in multiple conformational states.
  • HostingRepository: PRIDE
  • AnnounceDate: 2019-09-11
  • AnnouncementXML: Submission_2019-09-11_05:42:17.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original dataset
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Jason Williams
  • SpeciesList: scientific name: Chaetomium thermophilum; NCBI TaxID: 209285;
  • ModificationList: crosslinked residues; monohydroxylated residue
  • Instrument: Q Exactive

Dataset History

RevisionDatetimeStatusChangeLog Entry
02019-07-24 08:02:16ID requested
12019-09-11 05:42:18announced

Publication List

  1. Pillon MC, Hsu AL, Krahn JM, Williams JG, Goslen KH, Sobhany M, Borgnia MJ, Stanley RE, Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex. Nat Struct Mol Biol, 26(9):830-839(2019) [pubmed]

Keyword List

  1. submitter keyword: crosslink GRC3 Las1 BS3

Contact List

    Jason G. Williams
    • contact affiliation: Mass Spectrometry Research and Support Group Epigenetics and Stem Cell Biology Laboratory National Institute of Environmental Health Sciences 111 TW Alexander Drive MD F0-04 Research Triangle Park, North Carolina 27709
    • contact email: willia56@niehs.nih.gov
    • lab head:
    Jason Williams
    • contact affiliation: National Institute of Environmental Health Sciences
    • contact email: willia56@niehs.nih.gov
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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