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PXD014738 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBS3 crosslinking of GRC3 and Las1
DescriptionRibosome assembly is a complex process involving the coordination of multiple enzymes. A recently discovered endoribonuclease (RNase), Las1, and the poly-nucleotide kinase (PNK), Grc3, assemble into a complex. To attempt to understand the coordination of the two enzymes in this complex, we performed chemical crosslinking and mass spectrometry and also solved a series of cryo-electron microscopy structures of RNase PNK in multiple conformational states.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJason Williams
SpeciesList scientific name: Chaetomium thermophilum; NCBI TaxID: 209285;
ModificationListcrosslinked residues; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-24 08:02:16ID requested
12019-09-11 05:42:18announced
Publication List
Pillon MC, Hsu AL, Krahn JM, Williams JG, Goslen KH, Sobhany M, Borgnia MJ, Stanley RE, Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex. Nat Struct Mol Biol, 26(9):830-839(2019) [pubmed]
Keyword List
submitter keyword: crosslink GRC3 Las1 BS3
Contact List
Jason G. Williams
contact affiliationMass Spectrometry Research and Support Group Epigenetics and Stem Cell Biology Laboratory National Institute of Environmental Health Sciences 111 TW Alexander Drive MD F0-04 Research Triangle Park, North Carolina 27709
contact emailwillia56@niehs.nih.gov
lab head
Jason Williams
contact affiliationNational Institute of Environmental Health Sciences
contact emailwillia56@niehs.nih.gov
dataset submitter
Full Dataset Link List
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PRIDE project URI
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