PXD014738

PXD014738 is an original dataset announced via ProteomeXchange.

Title	BS3 crosslinking of GRC3 and Las1
Description	Ribosome assembly is a complex process involving the coordination of multiple enzymes. A recently discovered endoribonuclease (RNase), Las1, and the poly-nucleotide kinase (PNK), Grc3, assemble into a complex. To attempt to understand the coordination of the two enzymes in this complex, we performed chemical crosslinking and mass spectrometry and also solved a series of cryo-electron microscopy structures of RNase PNK in multiple conformational states.
HostingRepository	PRIDE
AnnounceDate	2019-09-11
AnnouncementXML	Submission_2019-09-11_05:42:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jason Williams
SpeciesList	scientific name: Chaetomium thermophilum; NCBI TaxID: 209285;
ModificationList	crosslinked residues; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-24 08:02:16	ID requested
⏵ 1	2019-09-11 05:42:18	announced

Pillon MC, Hsu AL, Krahn JM, Williams JG, Goslen KH, Sobhany M, Borgnia MJ, Stanley RE, Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex. Nat Struct Mol Biol, 26(9):830-839(2019) [pubmed]

submitter keyword: crosslink GRC3 Las1 BS3

Jason G. Williams
contact affiliation	Mass Spectrometry Research and Support Group Epigenetics and Stem Cell Biology Laboratory National Institute of Environmental Health Sciences 111 TW Alexander Drive MD F0-04 Research Triangle Park, North Carolina 27709
contact email	willia56@niehs.nih.gov
lab head
Jason Williams
contact affiliation	National Institute of Environmental Health Sciences
contact email	willia56@niehs.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014738

PRIDE project URI

• PRIDE
  1. PXD014738
     1. Label: PRIDE project
     2. Name: BS3 crosslinking of GRC3 and Las1