PXD014731-1

PXD014731 is an original dataset announced via ProteomeXchange.

Title	Aspartate residues far from the active site drive O-GlcNAc transferase substrate selection
Description	Here we use protein microarray technology and proteome-wide glycosylation profiling to show that conserved aspartate residues in the tetratricopeptide repeat (TPR) lumen of OGT drive substrate selection. Changing these residues to alanines alters substrate selectivity and unexpectedly increases rates of protein glycosylation. Our findings support a model where sites of glycosylation for many OGT substrates are determined by TPR domain contacts to substrate side chains five to fifteen residues Cterminal to the glycosite. In addition to guiding design of inhibitors that target OGT’s TPR domain, this information will inform efforts to engineer substrates to explore biological functions.
HostingRepository	PRIDE
AnnounceDate	2019-07-26
AnnouncementXML	Submission_2019-07-26_03:29:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Woo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-24 06:14:10	ID requested
⏵ 1	2019-07-26 03:29:25	announced
2	2019-08-08 03:22:10	announced	Updated publication reference for PubMed record(s): 31373491.

Dataset with its publication pending

submitter keyword: glycoproteomics, O-glcNAc

Christina Woo and Suzanne Walker
contact affiliation	Chemistry & Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138 and Department of Microbiology, Harvard Medical School, 4 Blackfan Circle, Boston, MA 02115
contact email	cwoo@chemistry.harvard.edu
lab head
Christina Woo
contact affiliation	Harvard University
contact email	cwoo@chemistry.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD014731

PRIDE project URI

• PRIDE
  1. PXD014731
     1. Label: PRIDE project
     2. Name: Aspartate residues far from the active site drive O-GlcNAc transferase substrate selection