PXD014648-1

PXD014648 is an original dataset announced via ProteomeXchange.

Title	Dose-dependent chemoresistance of serum-starved neuroblastoma cells against the anticancer drug
Description	Neuroblastoma is a solid, intractable, extracranial, pediatric tumor with complex, heterogeneous clinical behaviors. Chemotherapy is widely used to treat neuroblastoma and many studies have attempted to decipher the action mechanism of anticancer drugs; however, the dose-dependent responses of neuroblastoma cells to anticancer drugs in various microenvironments have not been fully explored. Here, we investigated the effects of different topotecan concentrations on human neuroblastoma SK-N-SH cells under varying nutrient supply conditions. The survival rate of serum-starved SK-N-SH cells increased upon treatment with a high concentration (1 μM) of topotecan. Using quantitative proteomics and network analysis, we showed that topotecan (1 μM) upregulated DNA repair and cholesterol-mediated topotecan efflux, resulting in topotecan resistance. Time-resolved topotecan quantitation using multiple reaction monitoring mass spectrometry further supports that topotecan resistance was associated with enhanced drug efflux. Our results provide a model for high-dose-dependent chemoresistance in neuroblastoma cells that could enable a patient-dependent chemotherapy screening strategy.
HostingRepository	PRIDE
AnnounceDate	2019-07-19
AnnouncementXML	Submission_2019-07-19_06:05:40.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014648
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dowoon Nam
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Deamidated; TMT6plex; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-18 01:16:41	ID requested
⏵ 1	2019-07-19 06:05:41	announced
2	2020-06-23 00:30:16	announced	2020-06-23: Updated project metadata.

Dataset with its publication pending

submitter keyword: Neuroblastoma, Serum starvation, Chemoresistance, Proteomics, Network model, Mass spectrometry

Sang-Won Lee
contact affiliation	Department of Chemistry, Korea University, Seoul 02841, Republic of Korea Center for Proteogenome Research, Korea University, Seoul 02841, Republic of Korea
contact email	sw_lee@korea.ac.kr
lab head
Dowoon Nam
contact affiliation	Korea University
contact email	dowoon0517@korea.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014648
     1. Label: PRIDE project
     2. Name: Dose-dependent chemoresistance of serum-starved neuroblastoma cells against the anticancer drug