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PXD014648 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDose-dependent chemoresistance of serum-starved neuroblastoma cells against the anticancer drug
DescriptionNeuroblastoma is a solid, intractable, extracranial, pediatric tumor with complex, heterogeneous clinical behaviors. Chemotherapy is widely used to treat neuroblastoma and many studies have attempted to decipher the action mechanism of anticancer drugs; however, the dose-dependent responses of neuroblastoma cells to anticancer drugs in various microenvironments have not been fully explored. Here, we investigated the effects of different topotecan concentrations on human neuroblastoma SK-N-SH cells under varying nutrient supply conditions. The survival rate of serum-starved SK-N-SH cells increased upon treatment with a high concentration (1 μM) of topotecan. Using quantitative proteomics and network analysis, we showed that topotecan (1 μM) upregulated DNA repair and cholesterol-mediated topotecan efflux, resulting in topotecan resistance. Time-resolved topotecan quantitation using multiple reaction monitoring mass spectrometry further supports that topotecan resistance was associated with enhanced drug efflux. Our results provide a model for high-dose-dependent chemoresistance in neuroblastoma cells that could enable a patient-dependent chemotherapy screening strategy.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDowoon Nam
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListDeamidated; TMT6plex; Oxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-18 01:16:41ID requested
12019-07-19 06:05:41announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Neuroblastoma, Serum starvation, Chemoresistance, Proteomics, Network model, Mass spectrometry
Contact List
Sang-Won Lee
contact affiliationDepartment of Chemistry, Korea University, Seoul 02841, Republic of Korea Center for Proteogenome Research, Korea University, Seoul 02841, Republic of Korea
contact emailsw_lee@korea.ac.kr
lab head
Dowoon Nam
contact affiliationKorea University
contact emaildowoon0517@korea.ac.kr
dataset submitter
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