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PXD014648

PXD014648 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHomologous recombination repair and cholesterol-mediated drug efflux induce dose-dependent chemoresistance in nutrient-deprived neuroblastoma cells
DescriptionNeuroblastoma is a solid pediatric tumor with heterogeneous clinical behaviors. Chemotherapy is widely used to treat neuroblastoma. However, dose-dependent responses and chemoresistance mechanisms of neuroblastoma cells to anticancer drugs remain challenges in various conditions. Here, we investigated the dose-dependent effects of topotecan on human neuroblastoma cells (SK-N-SH, SH-SY5Y, and SK-N-BE) under various nutrient supply conditions. Serum-starved human neuroblastoma cells showed reduced toxicity, and their survival rate increased upon treatment with a high concentration (1 μM) of topotecan. Quantitative proteomics identified approximately 10,000 proteins in SK-N-SH cells. Network analysis revealed that topotecan up-regulated DNA repair and cholesterol-mediated topotecan efflux, resulting in topotecan resistance. Results of cell cycle and quantitative analyses of membrane cholesterol supported the validity of these resistance factors and their applicability to all neuroblastoma cells investigated in this study. Our results provide a model for high dose-dependent chemoresistance in neuroblastoma cells that could enable a patient-dependent chemotherapy screening strategy.
HostingRepositoryPRIDE
AnnounceDate2020-06-23
AnnouncementXMLSubmission_2020-06-23_00:30:15.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD014648
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDowoon Nam
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListDeamidated; TMT6plex; Oxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-18 01:16:41ID requested
12019-07-19 06:05:41announced
22020-06-23 00:30:16announced2020-06-23: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Neuroblastoma, Serum starvation, Chemoresistance, Proteomics, Network model, Mass spectrometry
Contact List
Sang-Won Lee
contact affiliationDepartment of Chemistry, Korea University, Seoul 02841, Republic of Korea Center for Proteogenome Research, Korea University, Seoul 02841, Republic of Korea
contact emailsw_lee@korea.ac.kr
lab head
Dowoon Nam
contact affiliationKorea University
contact emaildowoon0517@korea.ac.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
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