PXD014648 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Homologous recombination repair and cholesterol-mediated drug efflux induce dose-dependent chemoresistance in nutrient-deprived neuroblastoma cells |
Description | Neuroblastoma is a solid pediatric tumor with heterogeneous clinical behaviors. Chemotherapy is widely used to treat neuroblastoma. However, dose-dependent responses and chemoresistance mechanisms of neuroblastoma cells to anticancer drugs remain challenges in various conditions. Here, we investigated the dose-dependent effects of topotecan on human neuroblastoma cells (SK-N-SH, SH-SY5Y, and SK-N-BE) under various nutrient supply conditions. Serum-starved human neuroblastoma cells showed reduced toxicity, and their survival rate increased upon treatment with a high concentration (1 μM) of topotecan. Quantitative proteomics identified approximately 10,000 proteins in SK-N-SH cells. Network analysis revealed that topotecan up-regulated DNA repair and cholesterol-mediated topotecan efflux, resulting in topotecan resistance. Results of cell cycle and quantitative analyses of membrane cholesterol supported the validity of these resistance factors and their applicability to all neuroblastoma cells investigated in this study. Our results provide a model for high dose-dependent chemoresistance in neuroblastoma cells that could enable a patient-dependent chemotherapy screening strategy. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-23 |
AnnouncementXML | Submission_2020-06-23_00:30:15.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD014648 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Dowoon Nam |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Deamidated; TMT6plex; Oxidation; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-18 01:16:41 | ID requested | |
1 | 2019-07-19 06:05:41 | announced | |
⏵ 2 | 2020-06-23 00:30:16 | announced | 2020-06-23: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Neuroblastoma, Serum starvation, Chemoresistance, Proteomics, Network model, Mass spectrometry |
Contact List
Sang-Won Lee |
contact affiliation | Department of Chemistry, Korea University, Seoul 02841, Republic of Korea Center for Proteogenome Research, Korea University, Seoul 02841, Republic of Korea |
contact email | sw_lee@korea.ac.kr |
lab head | |
Dowoon Nam |
contact affiliation | Korea University |
contact email | dowoon0517@korea.ac.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD014648 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014648
- Label: PRIDE project
- Name: Homologous recombination repair and cholesterol-mediated drug efflux induce dose-dependent chemoresistance in nutrient-deprived neuroblastoma cells