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PXD014425-2

PXD014425 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSilver and platinum nanoparticles synergy
DescriptionIn this study, we compared the effects of silver and platinum NPs on the main components of the blood brain barrier model: human cerebral microvascular endothelial cells (hCMEM/D3) and human primary astrocytes under single and co-exposure NP conditions. The co-exposure to two types of NPs synergistically inhibited proliferation of both cell types with the greater extent observed for endothelial cells (the combination index (CI) values for all tested concentrations were below 0.2 corresponding to strong or extreme synergism). In addition, NP-induced toxicity demonstrated dependence on the cell type with astrocytes being more tolerant to the metal NPs. The mechanism of synergy was further explored with short-time incubation time points (up to 30 min), where the cell metabolic activity was decreased to approximately 60% of the controls. The data obtained from the proteomics analysis suggests immunosuppression and deregulation of the extracellular matrix organization as well as contribution of the p75NTR-associated cell death executor (NADE)-dependent apoptotic mechanism in the synergistic cytotoxicity of NPs.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:17:42.644.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVladimir Gorshkov
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-28 06:51:36ID requested
12022-11-08 08:00:09announced
22023-11-14 08:17:43announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: platinum, astrocytes, blood brain barrier, endothelial cells,silver, nanoparticles, synergy, cytotoxicity
Contact List
Frank Kjeldsen
contact affiliationProfessor Department of Biochemistry and Molecular Biology University of Southern Denmark Campusvej 55 DK-5230 Odense M Denmark
contact emailfrankk@bmb.sdu.dk
lab head
Vladimir Gorshkov
contact affiliationUniversity of Southern Denmark
contact emailvgor@bmb.sdu.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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