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PXD014384 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMating precedes selective immune priming which is maintained throughout bumblebee queen diapause
DescriptionDiapause is a key life-history event characterised by arrested development, suppressed metabolism and increased stress tolerance. This state allows an organism to avoid prolonged periods of harsh and inhospitable environmental conditions. For species where only mated female adults undergo diapause, mating is performed prior to diapause. Mating can have a profound effect on the behaviour and physiology of females resulting in changes to the expression of key biological processes, including immunity. However, our understanding of how mating impacts long-term immunity and whether these effects persist throughout diapause is currently limited. Here we explored proteomic changes in the haemolymph of the ecologically important pollinator, Bombus terrestris. B. terrestris queens mate prior to diapause (a non-feeding arrest of development that can last 6-9 months). Using mass-spectrometry-based proteomics, we quantified changes in the pre-diapause queen haemolymph after mating, as well as the subsequent protein expression of mated queens during and post-diapause. Our results provide clear molecular evidence for the consequences and benefits of mating at the immune level through the selective increased abundance of antimicrobial peptides that are sustained throughout diapause. In addition our results provide novel insights into the molecular mechanisms by which bumblebees prepare for, survive, and recover from diapause, insights that may have implications for our general understanding of these processes in other insect groups.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Carolan
SpeciesList scientific name: Bombus terrestris audax; NCBI TaxID: 1255228;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-25 09:01:08ID requested
12019-11-18 06:10:18announced
22019-11-27 03:02:54announced2019-11-27: Updated project metadata.
32020-01-14 06:45:32announced2019-11-27: Updated project metadata.
2020-01-14: Updated publication reference for PubMed record(s): 31823732.
Publication List
Colgan TJ, Finlay S, Brown MJF, Carolan JC, Mating precedes selective immune priming which is maintained throughout bumblebee queen diapause. BMC Genomics, 20(1):959(2019) [pubmed]
Keyword List
submitter keyword: Bombus, diapause, AMPs, Proteomics
Contact List
James Carolan
contact affiliationDepartment of Biology, Maynooth University, Maynooth, Ireland
contact emailjames.c.carolan@gmail.com
lab head
James Carolan
contact affiliationBiology
contact emailjames.carolan@nuim.ie
dataset submitter
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