PXD014377-1
PXD014377 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Functional translatome proteomics reveal converging regulation by mTORC1 and eIF2α |
Description | Regulation of the translatome is essential during stress. However, the precise sets of translational targets regulated by the key translational stress responses –integrated stress response (ISR) and mTORC1 – remain elusive. We developed multiplexed enhanced protein dynamics (mePROD) proteomics, combining dynamic SILAC, multiplexing, and signal amplification, to enable measuring acute changes in protein synthesis. Treating cells with ISR/mTORC1-modulating stressors, we showed extensive translatome modulation and ~20% of proteins synthesized at highly reduced rates. Comparing translation-deficient sub-proteomes revealed an extensive overlap demonstrating that target specificity is achieved on protein level and not by pathway activation. Titrating inhibition of cap-dependent translation confirmed that synthesis of individual proteins is controlled by intrinsic properties responsive to global translation inhibition. This study reports a method to measure translation rates at the nascent chain level and provides insight into how the ISR and mTORC1, two key cellular pathways, regulate the translatome to guide cellular survival upon stress. |
HostingRepository | PRIDE |
AnnounceDate | 2019-12-06 |
AnnouncementXML | Submission_2019-12-06_10:07:27.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kevin Klann |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive HF; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-06-25 05:17:40 | ID requested | |
⏵ 1 | 2019-12-06 10:07:28 | announced | |
2 | 2019-12-09 04:03:59 | announced | 2019-12-09: Updated publication reference for DOI(s): doi.org/10.1016/j.molcel.2019.11.010. |
3 | 2020-01-15 03:45:54 | announced | 2019-12-09: Updated publication reference for DOI(s): doi.org/10.1016/j.molcel.2019.11.010. 2020-01-15: Updated publication reference for PubMed record(s): 31812349. 2020-01-15: Updated publication reference for DOI(s): doi.org/10.1016/j.molcel.2019.11.01 |
4 | 2023-11-14 08:50:18 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human, Translation, TMT, pulsed SILAC |
Contact List
Christian Münch | |
---|---|
contact affiliation | Universitätsklinikum Frankfurt am Main, Institut für Biochemie II |
contact email | ch.muench@em.uni-frankfurt.de |
lab head | |
Kevin Klann | |
contact affiliation | Universitätsklinikum Frankfurt am Main |
contact email | klann@em.uni-frankfurt.de |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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