PXD014377

PXD014377 is an original dataset announced via ProteomeXchange.

Title	Functional translatome proteomics reveal converging regulation by mTORC1 and eIF2α
Description	Regulation of the translatome is essential during stress. However, the precise sets of translational targets regulated by the key translational stress responses –integrated stress response (ISR) and mTORC1 – remain elusive. We developed multiplexed enhanced protein dynamics (mePROD) proteomics, combining dynamic SILAC, multiplexing, and signal amplification, to enable measuring acute changes in protein synthesis. Treating cells with ISR/mTORC1-modulating stressors, we showed extensive translatome modulation and ~20% of proteins synthesized at highly reduced rates. Comparing translation-deficient sub-proteomes revealed an extensive overlap demonstrating that target specificity is achieved on protein level and not by pathway activation. Titrating inhibition of cap-dependent translation confirmed that synthesis of individual proteins is controlled by intrinsic properties responsive to global translation inhibition. This study reports a method to measure translation rates at the nascent chain level and provides insight into how the ISR and mTORC1, two key cellular pathways, regulate the translatome to guide cellular survival upon stress.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:17.603.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kevin Klann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; isotope labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-25 05:17:40	ID requested
1	2019-12-06 10:07:28	announced
2	2019-12-09 04:03:59	announced	2019-12-09: Updated publication reference for DOI(s): doi.org/10.1016/j.molcel.2019.11.010.
3	2020-01-15 03:45:54	announced	2019-12-09: Updated publication reference for DOI(s): doi.org/10.1016/j.molcel.2019.11.010. 2020-01-15: Updated publication reference for PubMed record(s): 31812349. 2020-01-15: Updated publication reference for DOI(s): doi.org/10.1016/j.molcel.2019.11.01
⏵ 4	2023-11-14 08:50:18	announced	2023-11-14: Updated project metadata.

Klann K, Tascher G, M, ü, nch C, . Mol Cell, 77(4):913-925.e4(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, TMT, Translation, pulsed SILAC

Christian Münch
contact affiliation	Universitätsklinikum Frankfurt am Main, Institut für Biochemie II
contact email	ch.muench@em.uni-frankfurt.de
lab head
Kevin Klann
contact affiliation	Universitätsklinikum Frankfurt am Main
contact email	klann@em.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD014377

PRIDE project URI

• PRIDE
  1. PXD014377
     1. Label: PRIDE project
     2. Name: Functional translatome proteomics reveal converging regulation by mTORC1 and eIF2α