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PXD014280-1

PXD014280 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProfiling the surfaceome identifies therapeutic targets for cells with hyperactive mTORC1 signaling
DescriptionAberrantly high mTORC1 signaling is a known driver of many cancers and human disorders, yet pharmacological inhibition of mTORC1 rarely confers durable clinical responses. To explore alternative therapeutic strategies, herein we conducted a proteomics survey to identify cell surface proteins upregulated by mTORC1. A comparison of the surfaceome from Tsc1-/- versus Tsc1+/+ mouse embryonic fibroblasts revealed 59 proteins predicted to be significantly overexpressed in Tsc1-/- cells. Further validation of the data in multiple mouse and human cell lines showed that mTORC1 signaling most dramatically induced the expression of the proteases neprilysin (NEP/CD10) and aminopeptidase N (APN/CD13). Functional studies showed that constitutive mTORC1 signaling sensitized cells to genetic ablation of NEP and APN, as well as the biochemical inhibition of APN. In summary, these data show that mTORC1 signaling plays a significant role in the constitution of the surfaceome, which in turn may present novel therapeutic strategies.
HostingRepositoryMassIVE
AnnounceDate2020-01-15
AnnouncementXMLSubmission_2020-01-15_11:28:24.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKevin Leung
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; deglycosylated asparagine; 6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-17 19:16:56ID requested
12020-01-15 11:28:25announced
Publication List
no publication
Keyword List
submitter keyword: SILAC, TSC, mTORC1 singaling, Drug target
Contact List
Kevin K Leung
contact affiliationUCSF
contact emailkevin.leung@ucsf.edu
lab head
Kevin Leung
contact affiliationUCSF
contact emailkevin.leung@ucsf.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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