<<< Full experiment listing

PXD014199-1

PXD014199 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphorylation of multiple proteins involved in ciliogenesis by Tau Tubulin kinase 2
DescriptionAbstract Primary cilia are organelles necessary for proper implementation of developmental and homeostasis processes. To initiate their assembly, coordinated actions of multiple proteins are needed. Tau tubulin kinase 2 (TTBK2) is a key player in the cilium assembly pathway, controlling final step of cilia initiation. The function of TTBK2 in ciliogenesisis critically dependent on its kinase activity, however, precise mechanism of action of this kinase is so far incompletely understood, in part due to very limited information about its relevant substrates. In this study we identify CEP83, CEP89, CCDC92, Rabin8 and DVL3 as substrates of TTBK2 kinase activity. Further, we characterise a set of phosphosites of the newly identified substrates and CEP164, induced by TTBK2 in vitro and in vivo and show that TTBK2 preferentially phosphorylates examined substrates at intrinsically disordered regions (IDRs). Intriguingly, we further show that identified TTBK2 phosphosites and consensus sequence delineated from those are distinct from motifs previously assigned to TTBK2. Finally, we address functional relevance of selected phosphorylations of CEP164 and provide evidence that the examined TTBK2-induced phosphorylations of CEP164 are relevant for the process of cilia formation. In summary, our work provides important insight into substrates-TTBK2 kinase relationship and suggests that multiple phosphorylation of IDRs is probably involved in the control of ciliogenesis in human cells.
HostingRepositoryPRIDE
AnnounceDate2020-03-25
AnnouncementXMLSubmission_2020-03-25_05:39:09.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKaterina Hanáková
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-10 03:19:48ID requested
12020-03-25 05:39:11announced
Publication List
Bernatik O, Pejskova P, Vyslouzil D, Hanakova K, Zdrahal Z, Cajanek L, Phosphorylation of multiple proteins involved in ciliogenesis by Tau Tubulin kinase 2. Mol Biol Cell, 31(10):1032-1046(2020) [pubmed]
Keyword List
submitter keyword: TTBK2, phosphorylation, mass spectrometry, CEP164, CEP83, CEP89, CCDC92, Rabin8, DVL3
Contact List
Zbynek Zdrahal
contact affiliationNational Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic; Proteomics Core facility, Masaryk University, Brno, Czech Republic
contact emailzdrahal@sci.muni.cz
lab head
Katerina Hanáková
contact affiliationCEITEC MU
contact emailkaterina.hanak@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
[ + ]