PXD014199-1

PXD014199 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of multiple proteins involved in ciliogenesis by Tau Tubulin kinase 2
Description	Abstract Primary cilia are organelles necessary for proper implementation of developmental and homeostasis processes. To initiate their assembly, coordinated actions of multiple proteins are needed. Tau tubulin kinase 2 (TTBK2) is a key player in the cilium assembly pathway, controlling final step of cilia initiation. The function of TTBK2 in ciliogenesisis critically dependent on its kinase activity, however, precise mechanism of action of this kinase is so far incompletely understood, in part due to very limited information about its relevant substrates. In this study we identify CEP83, CEP89, CCDC92, Rabin8 and DVL3 as substrates of TTBK2 kinase activity. Further, we characterise a set of phosphosites of the newly identified substrates and CEP164, induced by TTBK2 in vitro and in vivo and show that TTBK2 preferentially phosphorylates examined substrates at intrinsically disordered regions (IDRs). Intriguingly, we further show that identified TTBK2 phosphosites and consensus sequence delineated from those are distinct from motifs previously assigned to TTBK2. Finally, we address functional relevance of selected phosphorylations of CEP164 and provide evidence that the examined TTBK2-induced phosphorylations of CEP164 are relevant for the process of cilia formation. In summary, our work provides important insight into substrates-TTBK2 kinase relationship and suggests that multiple phosphorylation of IDRs is probably involved in the control of ciliogenesis in human cells.
HostingRepository	PRIDE
AnnounceDate	2020-03-25
AnnouncementXML	Submission_2020-03-25_05:39:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katerina Hanáková
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-10 03:19:48	ID requested
⏵ 1	2020-03-25 05:39:11	announced

Bernatik O, Pejskova P, Vyslouzil D, Hanakova K, Zdrahal Z, Cajanek L, Phosphorylation of multiple proteins involved in ciliogenesis by Tau Tubulin kinase 2. Mol Biol Cell, 31(10):1032-1046(2020) [pubmed]

submitter keyword: TTBK2, phosphorylation, mass spectrometry, CEP164, CEP83, CEP89, CCDC92, Rabin8, DVL3

Zbynek Zdrahal
contact affiliation	National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic; Proteomics Core facility, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Katerina Hanáková
contact affiliation	CEITEC MU
contact email	katerina.hanak@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014199
     1. Label: PRIDE project
     2. Name: Phosphorylation of multiple proteins involved in ciliogenesis by Tau Tubulin kinase 2