PXD014070-2

PXD014070 is an original dataset announced via ProteomeXchange.

Title	Differential protein expression profiles of bronchoalveolar lavage fluid following lipopolysaccharide-induced direct and indirect lung injury in mice
Description	Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is an inflammatory process of the lungs characterized by increased permeability of the alveolar-capillary membrane with subsequent interstitial/alveolar edema and diffuse alveolar damage. ALI/ARDS can be the results of either direct or indirect lung injury, with pneumonia being the most common direct pulmonary insult and sepsis the most common extra-pulmonary cause. In this study, we employed the murine lipopolysaccharide (LPS)-induced direct and indirect lung injury model to explore the pathogenic mechanisms of pulmonary and extra-pulmonary ARDS, using an unbiased, discovery and quantitative proteomic approach. A total of 1,017 proteins were both identified and quantified in bronchoalveolar lavage fluid (BALF) from control, intratracheal LPS (I.T. LPS, 0.1 mg/kg) and intraperitoneal LPS (I.P. LPS, 5 mg/kg) treated mice. The two LPS groups shared 13 up-regulated and 22 down-regulated proteins compared to the control group. Among them, molecules related to bronchial and type II alveolar epithelial cell functions including cell adhesion molecule 1 and surfactant protein B were reduced, whereas lactotransferrin and resistin like alpha involved in lung innate immunity were upregulated in both LPS groups. Proteomic profiling also identified significant differences in BALF proteins between I.T. and I.P. LPS groups. Ingenuity pathway analysis revealed that acute-phase response signaling was activated by both I.T. and I.P. LPS, however, the magnitude of activation is much greater in I.T. LPS group compared to I.P. LPS group. Intriguingly, two canonical signaling pathways, liver X receptor/retinoid X receptor activation and the production of nitric oxide and reactive oxygen species in macrophages, were activated by I.T. LPS but suppressed by I.P. LPS. In addition, CXCL15 (also known as lungkine) was also up-regulated by I.T LPS but down-regulated by I.P. LPS. In conclusion, our quantitative discovery-based proteomic approach identified commonalities as well as significant differences in BALF protein expression profiles in LPS-induced direct and indirect lung injury, and importantly, LPS-induced indirect lung injury results in suppression of select components of lung innate immunity, which could contribute to the so-called “immunoparalysis” in sepsis patients.
HostingRepository	PRIDE
AnnounceDate	2019-07-11
AnnouncementXML	Submission_2019-08-15_00:00:55.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014070
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jessie Guidry
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Phospho; TMT6plex; Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-30 01:07:48	ID requested
1	2019-07-11 05:05:32	announced
⏵ 2	2019-08-15 00:02:27	announced	Updated publication reference for PubMed record(s): 31373289.

Yue X, Guidry JJ, Differential Protein Expression Profiles of Bronchoalveolar Lavage Fluid Following Lipopolysaccharide-Induced Direct and Indirect Lung Injury in Mice. Int J Mol Sci, 20(14):(2019) [pubmed]

submitter keyword: Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS), bronchoalveolar lavage fluid (BALF), murine lipopolysaccharide (LPS)-induced direct and indirect lung injury model

Jessie J Guidry
contact affiliation	The LSUHSC Proteomics Core3 Facility The Department of Biochemistry and Molecular Biology Louisiana State University Health Sciences Center New Orleans, LA 70112
contact email	jjguid@lsuhsc.edu
lab head
Jessie Guidry
contact affiliation	Louisiana State University Health Sciences Center Department of Biochemistry and Molecular Biology LSUHSC Proteomics Core Facility
contact email	jjguid@lsuhsc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014070
     1. Label: PRIDE project
     2. Name: Differential protein expression profiles of bronchoalveolar lavage fluid following lipopolysaccharide-induced direct and indirect lung injury in mice