PXD013861 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Endodermis-specific Receptor-like Kinase (ERK1) is required for the deposition of lignin in the Casparian strip in the root |
Description | The Casparian strip constitutes a physical diffusion barrier formed by the polar deposition of lignin in the root endodermis. The polar deposition of lignin is thought to be mediated by the scaffolding activity of membrane bound Casparian Strip domain proteins (CASPs) and the dirigent domain-containing protein Enhanced Suberin1 (ESB1). Here, we show that the endodermis-specific receptor-like kinase (ERK1), is part of this machinery, playing an essential role in the localization of CASP proteins and in the deposition of lignin, which ultimately are required for the formation of afunctional Casparian strip. ERK1 is localized to the cytoplasm and nucleus of the endodermis, and is part of a signalling pathway that implicates the circadian clock regulator Time for Coffee (TIC). In addition, we found that loss of ERK1 and TIC disrupts the Casparian strip organisation and alters composition of the root microbiome. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-08 |
AnnouncementXML | Submission_2021-09-08_09:49:49.887.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD013861 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Andrew Bottrill |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | Phospho; Oxidation; Carbamidomethyl |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-15 09:11:49 | ID requested | |
⏵ 1 | 2021-09-08 09:49:50 | announced | |
Publication List
Durr J, Reyt G, Spaepen S, Hilton S, Meehan C, Qi W, Kamiya T, Flis P, Dickinson HG, Feher A, Shivshankar U, Pavagadhi S, Swarup S, Salt D, Bending GD, Gutierrez-Marcos J, A Novel Signaling Pathway Required for Arabidopsis Endodermal Root Organization Shapes the Rhizosphere Microbiome. Plant Cell Physiol, 62(2):248-261(2021) [pubmed] |
Keyword List
submitter keyword: ERK1, Root, Arabidopsis, endodermis, phosphorylation, Time for Coffee |
Contact List
Dr Jose Gutierrez-Marcos |
contact affiliation | School of Life Sciences, University of Warwick, Coventry, United Kingdom |
contact email | J.F.Gutierrez-Marcos@warwick.ac.uk |
lab head | |
Andrew Bottrill |
contact affiliation | University of Warwick |
contact email | a.r.bottrill@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013861
- Label: PRIDE project
- Name: Endodermis-specific Receptor-like Kinase (ERK1) is required for the deposition of lignin in the Casparian strip in the root