PXD013861

PXD013861 is an original dataset announced via ProteomeXchange.

Title	Endodermis-specific Receptor-like Kinase (ERK1) is required for the deposition of lignin in the Casparian strip in the root
Description	The Casparian strip constitutes a physical diffusion barrier formed by the polar deposition of lignin in the root endodermis. The polar deposition of lignin is thought to be mediated by the scaffolding activity of membrane bound Casparian Strip domain proteins (CASPs) and the dirigent domain-containing protein Enhanced Suberin1 (ESB1). Here, we show that the endodermis-specific receptor-like kinase (ERK1), is part of this machinery, playing an essential role in the localization of CASP proteins and in the deposition of lignin, which ultimately are required for the formation of afunctional Casparian strip. ERK1 is localized to the cytoplasm and nucleus of the endodermis, and is part of a signalling pathway that implicates the circadian clock regulator Time for Coffee (TIC). In addition, we found that loss of ERK1 and TIC disrupts the Casparian strip organisation and alters composition of the root microbiome.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_09:49:49.887.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013861
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Phospho; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-15 09:11:49	ID requested
⏵ 1	2021-09-08 09:49:50	announced

Durr J, Reyt G, Spaepen S, Hilton S, Meehan C, Qi W, Kamiya T, Flis P, Dickinson HG, Feher A, Shivshankar U, Pavagadhi S, Swarup S, Salt D, Bending GD, Gutierrez-Marcos J, A Novel Signaling Pathway Required for Arabidopsis Endodermal Root Organization Shapes the Rhizosphere Microbiome. Plant Cell Physiol, 62(2):248-261(2021) [pubmed]

submitter keyword: ERK1, Root, Arabidopsis, endodermis, phosphorylation, Time for Coffee

Dr Jose Gutierrez-Marcos
contact affiliation	School of Life Sciences, University of Warwick, Coventry, United Kingdom
contact email	J.F.Gutierrez-Marcos@warwick.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD013861

PRIDE project URI

• PRIDE
  1. PXD013861
     1. Label: PRIDE project
     2. Name: Endodermis-specific Receptor-like Kinase (ERK1) is required for the deposition of lignin in the Casparian strip in the root