PXD013860 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Alterations in lipid profile of the aging kidney identified by MALDI imaging mass spectrometry |
Description | During aging, the kidney undergoes functional and physiological changes that are closely affiliated with chronic kidney disease (CKD). There is increasing evidence supporting the role of lipid or lipid-derived mediators in the pathogenesis of CKD and other aging-related diseases. To understand the role of lipids in various metabolic processes during kidney aging, we conducted MALDI imaging mass spectrometry (MALDI-IMS) analysis in kidneys harvested from young (2 months old, n=3) and old mice (24 months old, n=3). MALDI-IMS analysis showed an increase in ceramide level and a decrease in sphingomyelin (SM) and phosphatidylcholine (PC) levels in kidneys of old mice. The increased expression of cPLA2 and SMPD1 protein in aged kidney was confirmed by immunohistochemistry and western blot analysis. Our MALDI-IMS data showed the altered distribution of lipids in aged kidney as indicative of aging-related functional changes of the kidney. Combined analysis of MALDI-IMS and IHC confirmed lipidomic changes and expression levels of responsible enzymes as well as morphological changes. |
HostingRepository | PRIDE |
AnnounceDate | 2019-06-10 |
AnnouncementXML | Submission_2019-08-18_23:56:09.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joon Won Lee |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics flex series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-05-15 06:26:55 | ID requested | |
1 | 2019-06-10 04:08:18 | announced | |
2 | 2019-06-20 03:59:03 | announced | Updated publication reference for PubMed record(s): 31174782. |
⏵ 3 | 2019-08-18 23:56:10 | announced | Updated publication reference for PubMed record(s): 31174782. |
Publication List
Noh SA, Kim SM, Park SH, Kim DJ, Lee JW, Kim YG, Moon JY, Lim SJ, Lee SH, Kim KP, Alterations in Lipid Profile of the Aging Kidney Identified by MALDI Imaging Mass Spectrometry. J Proteome Res, 18(7):2803-2812(2019) [pubmed] |
Izquierdo-Gonz, á, lez JJ, Amil-Ruiz F, Zazzu S, S, á, nchez-Lucas R, Fuentes-Almagro CA, Rodr, í, guez-Ortega MJ, Proteomic analysis of goat milk kefir: Profiling the fermentation-time dependent protein digestion and identification of potential peptides with biological activity. Food Chem, 295():456-465(2019) [pubmed] |
Keyword List
submitter keyword: IMS, CKD, Chronic kidney disease, lipidomics, renal fibrosis |
Contact List
Kwang Pyo Kim |
contact affiliation | Department of Applied Chemistry, Institute of Natural Science, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin, Republic of Korea; Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul, Republic of Korea. |
contact email | kimkp@khu.ac.kr |
lab head | |
Joon Won Lee |
contact affiliation | Kyung Hee University |
contact email | leejw85@khu.ac.kr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013860
- Label: PRIDE project
- Name: Alterations in lipid profile of the aging kidney identified by MALDI imaging mass spectrometry