PXD013860

PXD013860 is an original dataset announced via ProteomeXchange.

Title	Alterations in lipid profile of the aging kidney identified by MALDI imaging mass spectrometry
Description	During aging, the kidney undergoes functional and physiological changes that are closely affiliated with chronic kidney disease (CKD). There is increasing evidence supporting the role of lipid or lipid-derived mediators in the pathogenesis of CKD and other aging-related diseases. To understand the role of lipids in various metabolic processes during kidney aging, we conducted MALDI imaging mass spectrometry (MALDI-IMS) analysis in kidneys harvested from young (2 months old, n=3) and old mice (24 months old, n=3). MALDI-IMS analysis showed an increase in ceramide level and a decrease in sphingomyelin (SM) and phosphatidylcholine (PC) levels in kidneys of old mice. The increased expression of cPLA2 and SMPD1 protein in aged kidney was confirmed by immunohistochemistry and western blot analysis. Our MALDI-IMS data showed the altered distribution of lipids in aged kidney as indicative of aging-related functional changes of the kidney. Combined analysis of MALDI-IMS and IHC confirmed lipidomic changes and expression levels of responsible enzymes as well as morphological changes.
HostingRepository	PRIDE
AnnounceDate	2019-06-10
AnnouncementXML	Submission_2019-08-18_23:56:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joon Won Lee
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics flex series

Revision	Datetime	Status	ChangeLog Entry
0	2019-05-15 06:26:55	ID requested
1	2019-06-10 04:08:18	announced
2	2019-06-20 03:59:03	announced	Updated publication reference for PubMed record(s): 31174782.
⏵ 3	2019-08-18 23:56:10	announced	Updated publication reference for PubMed record(s): 31174782.

Noh SA, Kim SM, Park SH, Kim DJ, Lee JW, Kim YG, Moon JY, Lim SJ, Lee SH, Kim KP, Alterations in Lipid Profile of the Aging Kidney Identified by MALDI Imaging Mass Spectrometry. J Proteome Res, 18(7):2803-2812(2019) [pubmed]

Izquierdo-Gonz, á, lez JJ, Amil-Ruiz F, Zazzu S, S, á, nchez-Lucas R, Fuentes-Almagro CA, Rodr, í, guez-Ortega MJ, Proteomic analysis of goat milk kefir: Profiling the fermentation-time dependent protein digestion and identification of potential peptides with biological activity. Food Chem, 295():456-465(2019) [pubmed]

submitter keyword: IMS, CKD, Chronic kidney disease, lipidomics, renal fibrosis

Kwang Pyo Kim
contact affiliation	Department of Applied Chemistry, Institute of Natural Science, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin, Republic of Korea; Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul, Republic of Korea.
contact email	kimkp@khu.ac.kr
lab head
Joon Won Lee
contact affiliation	Kyung Hee University
contact email	leejw85@khu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD013860

PRIDE project URI

• PRIDE
  1. PXD013860
     1. Label: PRIDE project
     2. Name: Alterations in lipid profile of the aging kidney identified by MALDI imaging mass spectrometry