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PXD013708

PXD013708 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChanges in the oligodendrocyte cell progenitor cell proteome with ageing
DescriptionFollowing CNS demyelination, adult oligodendrocyte progenitor cells (OPCs) can differentiate into new myelin-forming oligodendrocytes in a regenerative process called remyelination. While remyelination is very efficient in young adults, its efficiency declines progressively with ageing. Here we performed proteomic analysis on OPCs isolated acutely from the brains of neonate, young and aged female rats. Approximately 60% of the proteins are expressed at different levels in OPCs from neonates compared to their adult counterparts. The amount of myelin-associated and cell adhesion proteins are increased with age, while cholesterol-biosynthesis, transcription factors and cell cycle proteins decreased. Our experiments provide the first ageing OPC rodent proteome, revealing the distinct features of neonatal and adult OPCs, and providing new insights into why remyelination efficiency declines with ageing and potential roles for aged OPCs in other neurodegenerative diseases.
HostingRepositoryPRIDE
AnnounceDate2020-05-22
AnnouncementXMLSubmission_2020-05-22_05:43:04.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRayner Queiroz
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListdeaminated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-05-03 04:47:41ID requested
12020-05-22 05:43:05announced
Publication List
de la Fuente AG, Queiroz RML, Ghosh T, McMurran CE, Cubillos JF, Bergles DE, Fitzgerald DC, Jones CA, Lilley KS, Glover CP, Franklin RJM, Changes in the oligodendrocyte progenitor cell proteome with ageing. Mol Cell Proteomics, ():(2020) [pubmed]
Keyword List
submitter keyword: OPC ageing, proteomics, primary cells
Contact List
Prof. Robin J Franklin
contact affiliationWellcome-MRC Cambridge Stem Cell Institute Clifford Allbutt Building, Cambridge Biomedical Campus
contact emailrjf1000@cam.ac.uk
lab head
Rayner Queiroz
contact affiliationUniversity of Cambridge
contact emailrq214@cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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