PXD013605 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Co-utilization of sugar and aromatic carbons in Pseudomonas putida |
Description | Pseudomonas species thrive in different nutritional environments and can catabolize divergent carbon substrates. These capabilities have important implications for the role of these species in natural and engineered carbon processing. However, the metabolic phenotypes enabling Pseudomonas to utilize mixed substrates remain poorly understood. This work is part of a multi-omics approach involving stable isotope tracers, metabolomics, fluxomics, and proteomics in Pseudomonas putida KT2440 to investigate the constitutive metabolic network that achieves co-utilization of glucose and benzoate, respectively. The data used to estimate the changes in protein abundance are deposited here and were found to partially predicted the metabolic flux changes in cells grown on the glucose:benzoate mixture versus on glucose alone. Notably, flux magnitude and directionality were also maintained by metabolite levels and regulation of phosphorylation of key metabolic enzymes. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-08 |
AnnouncementXML | Submission_2021-09-08_09:37:19.392.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD013605 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ted Thannhauser |
SpeciesList | scientific name: Pseudomonas putida; NCBI TaxID: 303; |
ModificationList | Phospho; Deamidated; TMT6plex; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-23 05:46:20 | ID requested | |
⏵ 1 | 2021-09-08 09:37:21 | announced | |
Publication List
Mendonca CM, Yoshitake S, Wei H, Werner A, Sasnow SS, Thannhauser TW, Aristilde L, species. Proc Natl Acad Sci U S A, 117(51):32358-32369(2020) [pubmed] |
Keyword List
submitter keyword: Pseudomonas, bacterial metabolism, metabolomics, proteomics, glucose, benzoate, mixed-carbon metabolism, metabolic flux, metabolic regulation, fluxomics |
Contact List
Theodore Thannhauser |
contact affiliation | USDA-ARS |
contact email | tt34@cornell.edu |
lab head | |
Ted Thannhauser |
contact affiliation | USDA-ARS |
contact email | tt34@cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD013605 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013605
- Label: PRIDE project
- Name: Co-utilization of sugar and aromatic carbons in Pseudomonas putida