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PXD013605

PXD013605 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCo-utilization of sugar and aromatic carbons in Pseudomonas putida
DescriptionPseudomonas species thrive in different nutritional environments and can catabolize divergent carbon substrates. These capabilities have important implications for the role of these species in natural and engineered carbon processing. However, the metabolic phenotypes enabling Pseudomonas to utilize mixed substrates remain poorly understood. This work is part of a multi-omics approach involving stable isotope tracers, metabolomics, fluxomics, and proteomics in Pseudomonas putida KT2440 to investigate the constitutive metabolic network that achieves co-utilization of glucose and benzoate, respectively. The data used to estimate the changes in protein abundance are deposited here and were found to partially predicted the metabolic flux changes in cells grown on the glucose:benzoate mixture versus on glucose alone. Notably, flux magnitude and directionality were also maintained by metabolite levels and regulation of phosphorylation of key metabolic enzymes.
HostingRepositoryPRIDE
AnnounceDate2021-09-08
AnnouncementXMLSubmission_2021-09-08_09:37:19.392.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD013605
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTed Thannhauser
SpeciesList scientific name: Pseudomonas putida; NCBI TaxID: 303;
ModificationListPhospho; Deamidated; TMT6plex; Oxidation; Carbamidomethyl
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-04-23 05:46:20ID requested
12021-09-08 09:37:21announced
Publication List
Mendonca CM, Yoshitake S, Wei H, Werner A, Sasnow SS, Thannhauser TW, Aristilde L, species. Proc Natl Acad Sci U S A, 117(51):32358-32369(2020) [pubmed]
Keyword List
submitter keyword: Pseudomonas, bacterial metabolism, metabolomics, proteomics, glucose, benzoate, mixed-carbon metabolism, metabolic flux, metabolic regulation, fluxomics
Contact List
Theodore Thannhauser
contact affiliationUSDA-ARS
contact emailtt34@cornell.edu
lab head
Ted Thannhauser
contact affiliationUSDA-ARS
contact emailtt34@cornell.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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