PXD013489 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Connecting extracellular vesicle uptake and exosome biogenesis: intercellular communication in breast cancer cells is ITGB3-dependent |
Description | Metastasis, the spread of malignant cells from the primary tumour to distant sites is in 90% of the cases, the ultimate cause cancer related death. The integrin ITGB3 has been previously described to play an essential role in breast cancer metastasis, but the precise mechanisms remain undefined. We have now uncovered essential and thus far unknown roles of ITGB3 in vesicle uptake and exosome biogenesis. The functional requirement on ITGB3 is founded in its described interactions with heparan sulphated glyco-proteins (HSPGs) and the process of integrin recycling, allowing the capture of extracellular vesicles and their endocytosis-mediated internalisation. Key for the function of ITGB3 is the interaction and activation of the focal adhesion kinase (FAK), which is required for endocytosis of this vesicles and the entry of the endocytosis derived early endosomes into the exosome biogenesis pathway. Thus, ITGB3 has a central role in intracellular communication via extracellular vesicles, proposed to be critical for cancer metastasis. |
HostingRepository | PRIDE |
AnnounceDate | 2020-07-14 |
AnnouncementXML | Submission_2020-09-11_03:45:22.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eva Borràs |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-12 07:38:07 | ID requested | |
1 | 2020-07-14 02:42:52 | announced | |
⏵ 2 | 2020-09-11 03:45:23 | announced | 2020-09-11: Updated publication reference for PubMed record(s): 32848136. |
Publication List
Fuentes P, Ses, é M, Guijarro PJ, Emperador M, S, á, nchez-Redondo S, Peinado H, H, ü, mmer S, Ram, ó, n Y Cajal S, ITGB3-mediated uptake of small extracellular vesicles facilitates intercellular communication in breast cancer cells. Nat Commun, 11(1):4261(2020) [pubmed] |
Keyword List
submitter keyword: extracellular vesicle, breast cancer, ITGB3 |
Contact List
Eduard Sabido |
contact affiliation | Centre for Genomic Regulation (CRG),Barcelona, Spain |
contact email | eduard.sabido@crg.eu |
lab head | |
Eva Borràs |
contact affiliation | UPF |
contact email | eva.borras@upf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD013489 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013489
- Label: PRIDE project
- Name: Connecting extracellular vesicle uptake and exosome biogenesis: intercellular communication in breast cancer cells is ITGB3-dependent