PXD013351

PXD013351 is an original dataset announced via ProteomeXchange.

Title	Conversion of neonatal myocytes to pacemakers by viral transduction with Tbx18
Description	In this study, we performed two-dimensional liquid chromatography coupled to tandem mass spectrometry (2D-LC-MS/MS) to characterize the global transcriptome and proteome changes in Tbx18-iPMs compared to control, GFP-overexpressing neonatal rat ventricular myocytes (NRVMs). 67% of protein abundances changed significantly (4,475 of 6,661 proteins; p<0.05) at a false discovery rate of 1.6%. Downregulation was broad among metabolic pathways, such as TCA cycle, oxidative phosphorylation, glycolysis and fatty acid oxidation. Ion channels associated with ventricular excitation-contraction coupling (sodium, calcium, and potassium channels, Connexin 43, among others) were likewise downregulated. Pacemaker phenotype was characterized by upregulation of the pacemaker current channel (HCN4) but also mechnosensitive Piezo1, Trp channels Trpp2 (PKD2) and TrpM7, and Connexin45. Consistent with emergent pacemaker morphology, there was broad upregulation of cytoskeletal proteins and their small GTPase regulators. Extracellular matrix and growth factor/cytokine/interferon genes were also dynamically upregulated, indicating that Tbx18-iPMs are primed for sinoatrial node integration.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:06.872.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD013351
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	D. Brian Foster
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	Deamidated; TMT6plex; Oxidation; Carbamidomethyl; Gln->pyro-Glu
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-03 01:47:07	ID requested
1	2022-08-25 18:40:17	announced
⏵ 2	2023-11-14 08:50:07	announced	2023-11-14: Updated project metadata.

10.6019/PXD013351;

submitter keyword: Rat, TMT, neonatal rat ventricular myocytes, LC-MS/MS

D. Brian Foster and Hee Cheol Cho, corresponding authors
contact affiliation	Johns Hopkins School of Medicine & Emory University
contact email	dbrianfoster@jhmi.edu
lab head
D. Brian Foster
contact affiliation	Johns Hopkins University School of Medicine
contact email	dbrianfoster@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013351
     1. Label: PRIDE project
     2. Name: Conversion of neonatal myocytes to pacemakers by viral transduction with Tbx18