PXD013341 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling |
Description | Tau is a scaffolding protein which serves multiple cellular functions that are perturbed in neurodegenerative diseases, including Alzheimer’s disease (AD) and frontotemporal dementia (FTD). We have recently shown that amyloid-, the second hallmark of AD, induces de novo protein synthesis of tau. Importantly, this activation was found to be tau-dependent, raising the question of whether FTD-tau by itself affects protein synthesis. To investigate this, we applied non-canonical amino acid labelling to visualise and identify newly synthesised proteins in the K369I tau transgenic K3 mouse model of FTD. This revealed that protein synthesis was massively decreased in neurons containing pathologically phosphorylated tau, a finding confirmed in P301L mutant tau transgenic rTg4510 mice. Using quantitative SWATH-MS proteomics, we identified changes in 247 proteins of the de novo proteome of K3 mice. These included decreased synthesis of the ribosomal proteins RPL23, RPLP0, RPL19 and RPS16, a finding that was validated in both K3 and rTg4510 mice. Together, our findings present a potential pathomechanism by which pathological tau interferes with cellular functions through the dysregulation of ribosomal protein synthesis. |
HostingRepository | PRIDE |
AnnounceDate | 2019-05-15 |
AnnouncementXML | Submission_2019-05-15_00:44:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Harrison Evans |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-04-02 06:45:00 | ID requested | |
⏵ 1 | 2019-05-15 00:44:26 | announced | |
2 | 2019-05-31 02:36:03 | announced | Updated publication reference for PubMed record(s): 31118249. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Non-canonical amino acid labelling, tauopathy, protein synthesis, ribosomal proteins, neurodegenerative disease. |
Contact List
Professor Jürgen Götz |
contact affiliation | Clem Jones Centre of Aging Dementia Research, Queensland Brain Institute, University of Queensland |
contact email | j.goetz@uq.edu.au |
lab head | |
Harrison Evans |
contact affiliation | The University of Queensland |
contact email | h.evans@uq.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD013341
- Label: PRIDE project
- Name: Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling