PXD013341

PXD013341 is an original dataset announced via ProteomeXchange.

Title	Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling
Description	Tau is a scaffolding protein which serves multiple cellular functions that are perturbed in neurodegenerative diseases, including Alzheimer’s disease (AD) and frontotemporal dementia (FTD). We have recently shown that amyloid-, the second hallmark of AD, induces de novo protein synthesis of tau. Importantly, this activation was found to be tau-dependent, raising the question of whether FTD-tau by itself affects protein synthesis. To investigate this, we applied non-canonical amino acid labelling to visualise and identify newly synthesised proteins in the K369I tau transgenic K3 mouse model of FTD. This revealed that protein synthesis was massively decreased in neurons containing pathologically phosphorylated tau, a finding confirmed in P301L mutant tau transgenic rTg4510 mice. Using quantitative SWATH-MS proteomics, we identified changes in 247 proteins of the de novo proteome of K3 mice. These included decreased synthesis of the ribosomal proteins RPL23, RPLP0, RPL19 and RPS16, a finding that was validated in both K3 and rTg4510 mice. Together, our findings present a potential pathomechanism by which pathological tau interferes with cellular functions through the dysregulation of ribosomal protein synthesis.
HostingRepository	PRIDE
AnnounceDate	2019-05-17
AnnouncementXML	Submission_2019-05-31_02:36:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harrison Evans
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-04-02 06:45:00	ID requested
1	2019-05-15 00:44:26	announced
⏵ 2	2019-05-31 02:36:03	announced	Updated publication reference for PubMed record(s): 31118249.

submitter keyword: Non-canonical amino acid labelling, tauopathy, protein synthesis, ribosomal proteins, neurodegenerative disease.

Professor Jürgen Götz
contact affiliation	Clem Jones Centre of Aging Dementia Research, Queensland Brain Institute, University of Queensland
contact email	j.goetz@uq.edu.au
lab head
Harrison Evans
contact affiliation	The University of Queensland
contact email	h.evans@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD013341

PRIDE project URI

• PRIDE
  1. PXD013341
     1. Label: PRIDE project
     2. Name: Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling