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PXD013341

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-05-15
  • AnnouncementXML: Submission_2019-05-15_00:44:26.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Harrison Evans
  • Title: Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling
  • Description: Tau is a scaffolding protein which serves multiple cellular functions that are perturbed in neurodegenerative diseases, including Alzheimer’s disease (AD) and frontotemporal dementia (FTD). We have recently shown that amyloid-, the second hallmark of AD, induces de novo protein synthesis of tau. Importantly, this activation was found to be tau-dependent, raising the question of whether FTD-tau by itself affects protein synthesis. To investigate this, we applied non-canonical amino acid labelling to visualise and identify newly synthesised proteins in the K369I tau transgenic K3 mouse model of FTD. This revealed that protein synthesis was massively decreased in neurons containing pathologically phosphorylated tau, a finding confirmed in P301L mutant tau transgenic rTg4510 mice. Using quantitative SWATH-MS proteomics, we identified changes in 247 proteins of the de novo proteome of K3 mice. These included decreased synthesis of the ribosomal proteins RPL23, RPLP0, RPL19 and RPS16, a finding that was validated in both K3 and rTg4510 mice. Together, our findings present a potential pathomechanism by which pathological tau interferes with cellular functions through the dysregulation of ribosomal protein synthesis.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: TripleTOF 5600

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-04-02 06:45:00ID requested
12019-05-15 00:44:26announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. submitter keyword: Non-canonical amino acid labelling, tauopathy, protein synthesis, ribosomal proteins, neurodegenerative disease.

Contact List

    Professor Jürgen Götz
    • contact affiliation: Clem Jones Centre of Aging Dementia Research, Queensland Brain Institute, University of Queensland
    • contact email: j.goetz@uq.edu.au
    • lab head:
    Harrison Evans
    • contact affiliation: The University of Queensland
    • contact email: h.evans@uq.edu.au
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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